Emerging evidence indicates that dietary n‐3 polyunsaturated fatty acids (PUFA) alter the fatty acid composition of corpus luteum (CL) and directly affect the luteal function in the cow, which is independent of the inhibitory effect on the endometrial PGF_2α production. The present study, thus, investigated the effects of n‐3 PUFA rich fish oil (FO) supplementation on the transcriptional modulation of genes involved in the biosynthesis of progesterone (P₄) in the CL collected during the luteolytic phase of oestrous cycle in the goat. On the day of synchronized oestrus, goats (n = 6/group) were fed an isocaloric diet supplemented with either FO or palm oil (PO). The dose of oil supplementation was 0.6 mlkg^‐1 body weight, and the duration was 55–57 days. The FO provided 156 mgkg^‐1 body weight of n‐3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The CL was collected by laparotomy on day 16 post‐oestrus, and the relative abundance of P450 side‐chain cleaving enzyme, steroid acute regulatory protein (StAR) and 3β‐hydroxy steroid dehydrogenase (3β‐HSD) genes was quantitated by real‐time PCR. The results indicated that the dietary FO significantly upregulated the expression of 3β‐HSD by 1.13‐fold and downregulated StAR by ~2‐fold as compared to PO group (p < .05). It is concluded that dietary FO differently affected the expression of genes involved in P₄ synthesis in the CL during the luteolytic window of the oestrous cycle in the goat.

中文翻译：

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日粮n-3多不饱和脂肪酸的补充对山羊黄体（Capra hircus）孕酮生物合成相关基因表达的影响。

新兴证据表明，饮食中的n-3多不饱和脂肪酸（PUFA）会改变黄体（CL）的脂肪酸组成，并直接影响母牛的黄体功能，这与对子宫内膜_PGF2α产生的抑制作用无关。因此，本研究调查了富含n-3 PUFA的鱼油（FO）对发情周期黄体溶解期黄体溶解期收集的CL中与孕酮（P ₄）生物合成有关的基因转录调控的影响。山羊。在发情期当天，给山羊（ 每组n = 6）喂食补充了FO或棕榈油（PO）的等热量饮食。补充油的剂量为0.6 mlkg ^-1体重，持续时间为55-57天。FO提供了156 mgkg ^-1体重的n-3二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）。在发情后第16天通过剖腹术收集CL，并通过实时定量P450侧链裂解酶，类固醇急性调节蛋白（StAR）和3β-羟基类固醇脱氢酶（3β-HSD）基因的相对丰度。 PCR。结果表明，与PO组相比，饮食FO显着上调了3β-HSD的表达量1.13倍，而StAR则下调了约2倍（p  <.05）。结论是，在山羊雌性周期的黄体溶解窗口期间，饮食中的FO对CL中参与P ₄合成的基因的表达有不同的影响。