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A Novel, Efficient Method for Otolith Specimen Preparation Using UV‐Cured Resins
North American Journal of Fisheries Management ( IF 1.1 ) Pub Date : 2020-06-28 , DOI: 10.1002/nafm.10484
Carlos Augusto Strüssmann 1 , Kaho Miyoshi 1 , Shota Mitsui 1
Affiliation  

Otoliths are calcified structures that are located in the inner ears of fish, as in most vertebrates, that are responsible primarily for the perception of gravity, balance, and movement and secondarily of sound detection. Microstructural and chemical analyses of the inner otolith growth layers, called increments, constitute powerful tools for estimating fish age and elucidate many life history and demographic traits of fish populations. Otolith analyses often require the production of a thin cross section that includes the otolith core and all increments formed from birth (core) until the moment of collection (otolith edge) in the same plane of view. Here we report on the suitability of single‐component UV‐cured resins to obtain otolith cross sections for both microstructural examination and chemical analysis by electron probe microanalysis. Embedding blocks that were produced with UV‐cured resins had transparency that was comparable to that of the commonly used two‐component epoxy resins and provided similar adhesion and mechanical support for the otoliths during grinding or cutting with a precision cutter and analysis. In addition, UV‐cured resins offer on‐demand, extremely rapid (minute‐order) hardening that makes them useful in classroom situations in which time for otolith embedding becomes a constraint.

中文翻译:

一种使用紫外线固化树脂制备耳石样品的高效新方法

耳石是钙化的结构,像大多数脊椎动物一样,位于鱼的内耳中,主要负责重力,平衡和运动的感知,其次是声音检测。内耳石生长层的微观结构和化学分析(称为增量)是估算鱼类年龄并阐明鱼类种群的许多生活史和人口统计学特征的有力工具。耳石的分析通常需要产生一个薄的横截面,其中包括耳石的核以及从出生(核)到收集的瞬间(耳石边缘)在同一视平面上形成的所有增量。在这里,我们报告了单组分紫外线固化树脂是否适合获得耳石横截面,以进行显微结构检查和通过电子探针显微分析进行化学分析的适用性。用紫外线固化树脂生产的嵌块的透明度可与常用的两组分环氧树脂相媲美,并且在用精密切割机进行研磨或切割和分析过程中,对耳石的粘附性和机械支撑性相似。此外,紫外线固化树脂可按需提供极快的(分钟级)硬化,使其在教室环境中有用,因为在这种情况下,耳石嵌入的时间成为限制。
更新日期:2020-06-28
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