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Evaluation of functionality of type II toxin-antitoxin systems of Clostridioides difficile R20291.
Microbiological Research ( IF 6.7 ) Pub Date : 2020-06-28 , DOI: 10.1016/j.micres.2020.126539
R Álvarez 1 , C Ortega-Fuentes 1 , C Queraltó 1 , O Inostroza 1 , F Díaz-Yáñez 2 , R González 1 , I L Calderón 3 , J A Fuentes 4 , D Paredes-Sabja 2 , F Gil 2
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Clostridioides difficile is a nosocomial, Gram-positive, strictly anaerobic, spore-forming pathogen capable of colonizing and proliferating in the human intestine. In bacteria, it has been shown that the Toxin-Antitoxin systems mediate the cellular response to external stress by initiating processes such as biofilm formation and programmed cell death. This work aims to evaluate the functionality of four type II TA modules of Clostridioides difficile R20291. We performed bioinformatic analysis to search for putative TA systems using the TADB platform. Then we performed a heterologous expression assay to evaluate the functionality of these systems. Our results showed that the MazEF and RelBE systems were functional, suggesting that their corresponding toxins possess an endoribonuclease activity. In conclusion, MazEF and RelBE systems of C. difficile R20291 are functional in a heterologous expression system.



中文翻译:

艰难梭菌R20291的II型毒素-抗毒素系统的功能评估。

艰难梭菌是一种能够在人的肠道中定殖和繁殖的医院内革兰氏阳性,严格厌氧,可形成孢子的病原体。在细菌中,已经表明毒素-抗毒素系统通过启动诸如生物膜形成和程序性细胞死亡的过程来介导细胞对外部压力的反应。这项工作旨在评估艰难梭菌的四个II型TA模块的功能R20291。我们进行了生物信息学分析,以使用TADB平台搜索推定的TA系统。然后,我们进行了异源表达测定以评估这些系统的功能。我们的结果表明MazEF和RelBE系统具有功能,表明它们相应的毒素具有核糖核酸内切酶活性。总之,艰难梭菌R20291的MazEF和RelBE系统在异源表达系统中起作用。

更新日期:2020-06-28
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