The present study aims to investigate the endophytic bacteria, isolated from the roots of Taxus wallichiana Zucc. and designated as GBPI_TWL and GBPI_TWr, for their plant growth promoting traits. On the basis of phenotypic and molecular characters, the bacteria are identified as species of Burkholderia and Enterobacter, respectively. Both the bacteria could grow at wide range of temperature (5−40 °C, opt=25 °C) and pH (1.5–11.0, opt = 6–7), and tolerate salt concentration up to 12 %. While both the bacterial endophytes possessed siderophore, HCN, ammonia, and salicyclic acid producing abilities, GBPI_TWL showed IAA and ACC deaminase producing abilities, in addition. The bacteria were found to be potential phosphate solubilizers at wide temperature range (5−35 °C) by utilizing tricalcium, iron, and aluminium phosphate as substrate. Further, the bacterial isolates produced phytase and phosphatase enzymes in both acidic and alkaline conditions. Positive influence of the inoculation with the bioformulations of GBPI_TWL and GBPI_TWr was demonstrated on the test crops namely rice (Oryza sativa) and soybean (Glycine max) with respect to physico-chemical and plant growth parameters in net house experiments. The study will have implications in developing bioformulations, specifically for low temperature environments, in view of environmental sustainability.

本研究旨在调查从南方红豆杉根中分离出的内生细菌。因其促进植物生长的性状而命名为GBPI_TWL和GBPI_TWr。根据表型和分子特征，将细菌鉴定为伯克霍尔德氏菌和肠杆菌属， 分别。两种细菌都可以在很宽的温度范围（5-40°C，opt = 25°C）和pH（1.5-11.0，opt = 6-7）下生长，并能耐受高达12％的盐浓度。虽然这两种细菌内生菌都具有铁载体，HCN，氨和水杨酸生成能力，但GBPI_TWL还显示出IAA和ACC脱氨酶生成能力。通过使用磷酸三钙，铁和铝作为底物，发现该细菌是在宽温度范围（5-35°C）下潜在的磷酸盐增溶剂。此外，细菌分离物在酸性和碱性条件下均产生植酸酶和磷酸酶。GBPI_TWL和GBPI_TWr生物制剂的接种对试验作物即水稻（Oryza sativa）和大豆（在温室实验中有关物理化学和植物生长参数的甘氨酸最大值）。考虑到环境的可持续性，该研究将对开发生物制剂，特别是针对低温环境的生物制剂产生影响。