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Characterization of the fluorescence intensity and color tonality in the exoskeleton of scorpions.
Journal of Photochemistry and Photobiology B: Biology ( IF 5.4 ) Pub Date : 2020-06-28 , DOI: 10.1016/j.jphotobiol.2020.111945
Daniel López-Cabrera 1 , Gabriel Ramos-Ortiz 1 , Edmundo González-Santillán 2 , Rafael Espinosa-Luna 1
Affiliation  

Scorpions are a mesodiverse and nocturnal group of arachnids inhabiting most biomes worldwide. Different species of scorpions have divergent adaptations to the substrate they live in, but most of them share an intriguing characteristic: their exoskeleton contains fluorophores that emit blue-greenish fluorescence under ultraviolet radiation. Although there are some reports in the literature on the study of fluorescence in scorpions, the biological functionality of this light emission is currently unknown and is under debate. In this work, the properties of emission from the scorpion's exoskeleton are studied by means of digitally processed photographs taken of living specimens under ultraviolet illumination and complemented with standard spectroscopic measurements of emission and excitation spectra. With the aim of identifying possible correlations between the fluorescence, the characteristics color of the exoskeleton and the biology of the scorpions, 4 families, 9 genera and 24 species were studied. Our results suggest that the intensity of fluorescence is heterogeneous throughout the scorpion's exoskeleton studied here in such a way that pedipalps and metasomal segments fluoresce more intensely than the mesosomal segments. The spectrum of fluorescence across species is practically identical, suggesting that the same fluorophores are present in their exoskeletons. However, the fluorescence intensity emitted by each species varies according to their characteristic color (associated with the exoskeleton optical reflectance). Since the coloration of the exoskeleton is determined by the concentration of melanin and other pigments according to the substrate where scorpions live in, we conclude by suggesting that fluorescence may correlate directly to the ecomorphotype of the scorpions.



中文翻译:

蝎子外骨骼中荧光强度和颜色色调的特征。

蝎是生活在世界上大多数生物群落中的蜘蛛群的夜生活。不同种类的蝎子对它们所生活的基质有不同的适应性,但大多数蝎子都有一个吸引人的特征:它们的外骨骼含有在紫外线辐射下发出蓝绿色荧光的荧光团。尽管在文献中有一些关于蝎子中荧光研究的报道,但是这种光发射的生物学功能目前仍是未知的,并且还在争论中。在这项工作中,通过数字化处理活体标本在紫外线照射下的照片,并结合标准的发射光谱和激发光谱测量,研究了蝎子外骨骼的发射特性。为了确定荧光,外骨骼的特征颜色和蝎子的生物学之间可能的相关性,研究了4个科,9个属和24个种。我们的结果表明,在这里研究的整个蝎子的外骨骼中,荧光强度是异质的,这样的方式是,足尖和变体段的荧光强度要比中间体段的荧光强度高。跨物种的荧光光谱实际上是相同的,这表明在其外骨骼中存在相同的荧光团。但是,每种物质发出的荧光强度根据其特征颜色(与外骨骼光学反射率相关)而变化。

更新日期:2020-07-03
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