The skin keeps the human body healthy from extrinsic stimuli such as ultraviolet (UV) irradiation. However, chronic exposure of these stimuli reduces the number of proteins that constitute the extracellular matrix (ECM) and causes wrinkle formation. The amount of collagen, the main protein that constitutes connective tissue, is reduced in the human skin due to UV radiation. When human dermal fibroblasts were damaged by UVB, UVB increased the MMPs expressions and degraded type I collagen and other ECM proteins. Oyster (Crassostrea gigas) hydrolysate (OH) is known to have anticancer, antioxidant, and anti-apoptotic effects. To scrutinize the anti-wrinkle effect of the OH in the viewpoint of the balance between collagen degradation and synthesis, we conducted the study in UVB damaged human dermal fibroblasts. We determined type I procollagen, MMPs and related proteins using ELISA kit, qRT-PCR and western blot. In our study, we discovered that OH inhibits collagen degradation by regulating MAPKs, AP-1 and MMPs expression. Also, we found that OH promotes collagen production by enhancing TGFβ receptor II expression and Smad3 phosphorylation. These results showed that OH regulates collagen degradation and stimulates collagen synthesis. Through this study, we found that OH is effective in inhibiting wrinkle formation and restore photo-aged human skin. It indicates that OH can be one of the functional materials in the fields of anti-wrinkle research.

皮肤使人体免受紫外线（UV）等外在刺激。但是，这些刺激的长期暴露会减少构成细胞外基质（ECM）的蛋白质数量，并导致皱纹形成。胶原蛋白（构成结缔组织的主要蛋白质）的量由于紫外线辐射而在人的皮肤中减少。当人皮肤成纤维细胞被UVB破坏时，UVB会增加MMPs表达并降解I型胶原蛋白和其他ECM蛋白。牡蛎（Crassostrea gigas）水解物（OH）已知具有抗癌，抗氧化和抗凋亡作用。为了从胶原蛋白降解和合成之间的平衡的角度研究OH的抗皱效果，我们在UVB损伤的人类皮肤成纤维细胞中进行了研究。我们使用ELISA试剂盒，qRT-PCR和Western blot测定了I型原胶原，MMP和相关蛋白。在我们的研究中，我们发现OH通过调节MAPK，AP-1和MMP的表达来抑制胶原蛋白的降解。此外，我们发现OH通过增强TGFβ受体II表达和Smad3磷酸化来促进胶原蛋白的产生。这些结果表明，OH调节胶原蛋白降解并刺激胶原蛋白合成。通过这项研究，我们发现OH可有效抑制皱纹形成并恢复光老化的人类皮肤。