An increasing amount of Fluoranthene (Fla) and Benz(a)anthracene (BaA) is being produced and used, eventually entering the soil sediments. The accumulation of Fla and BaA will cause poisoning to typical enzymes (α-Amylase) and organisms (Eisenia fetida) in soil. However, the studies about exploring and comparing the different effects of Fla, BaA and their joint effect at different levels are rarely reported. In this paper, the different effects of Fla, BaA and their mixed pollutant on α-Amylase were evaluated and compared at the molecular level, and the effect of Fla-BaA to the antioxidant system of earthworm (Eisenia fetida) was investigated from the aspects of concentration and exposure time at the animal level. The results showed that Fla-BaA had the greatest influence on the skeleton structure and the microenvironment of amino acid residue of α-Amylase compared to Fla and BaA, and in the mixed pollutant system, the joint effect mode was additive mode. The inhibitory effect of Fla-BaA on the activity of α-Amylase was also stronger than that of the system alone. The assays at the animal level showed that low concentrations (below 5 mg/kg) of Fla-BaA increased the activity of GSH-Px and SOD while high concentrations inhibited their activity. The POD that was activated throughout the experiment period suggested its key role in the earthworm antioxidant system. Changes in T-AOC and MDA showed that long-term and high-dose of Fla-BaA exposure inhibited the antioxidant capacity of Eisenia fetida, causing lipid peroxidation and damage to cells.

越来越多的荧蒽（Fla）和苯并蒽（BaA）正在生产和使用，最终进入土壤沉积物中。Fla和BaA的积累会中毒土壤中的典型酶（α-淀粉酶）和生物（Eisenia fetida）。但是，很少有关于探索和比较Fla，BaA的不同作用及其在不同水平上的联合作用的研究。本文在分子水平上评价和比较了Fla，BaA及其混合污染物对α-淀粉酶的不同作用，并研究了Fla-BaA对earth抗氧化系统的影响。从动物水平上的浓度和暴露时间方面进行了研究。结果表明，与Fla和BaA相比，Fla-BaA对α-淀粉酶的骨架结构和氨基酸残基的微环境影响最大，在混合污染物体系中，联合效应模式为加和模式。Fla-BaA对α-淀粉酶活性的抑制作用也强于单独的系统。在动物水平上的分析表明，低浓度（低于5 mg / kg）的Fla-BaA会增加GSH-Px和SOD的活性，而高浓度会抑制其活性。在整个实验期间被激活的POD表明了其在worm抗氧化剂系统中的关键作用。赤子香，引起脂质过氧化和细胞损伤。