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Using bimetallic Au@Pt nanozymes as a visual tag and as an enzyme mimic in enhanced sensitive lateral-flow immunoassays: application for the detection of streptomycin
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.aca.2020.06.009
Dali Wei 1 , Xuyun Zhang 2 , Bin Chen 2 , Kun Zeng 2
Affiliation  

Because of the advantages of simplicity, cost-effectiveness and visibility, lateral-flow immunoassays (LFAs) have been widely used in the food safety field. However, the low sensitivity of LFAs needs to be solved. Nanozymes have amazing potential for application in biosensors due to their excellent and abundant enzyme-like characteristics. In this study, an Au@Pt nanozyme synthesized by a one-step method showed the higher affinity with TMB/H2O2 and higher catalytic efficiency than that of horseradish peroxidase (HRP). For the detection of streptomycin (STR), a typical aminoglycoside antibiotic, a novel LFA based on Au@Pt as a visual tag and an enhanced LFA based on the enzyme-like activity of Au@Pt by addition of the chromogenic substrate 3-amino-9-ethyl-carbazole (AEC) were established and compared with conventional LFA based on AuNPs. The qualitative limit of detection (LOD) was 1 ng mL-1 for the LFA based on Au@Pt as the visual tag and 0.1 ng mL-1 for the enhanced LFA based on the activity of Au@Pt, in comparison to 8 ng mL-1 for LFA based on AuNPs. Furthermore, the level of streptomycin in milk samples from Zhenjiang City was successfully evaluated by the novel LFA based on Au@Pt nanozyme. These results suggest that LFAs based on nanozymes are a promising and effective tool for food safety.

中文翻译:

在增强的灵敏侧流免疫测定中使用双金属 Au@Pt 纳米酶作为视觉标签和酶模拟物:在链霉素检测中的应用

由于具有简单、成本效益和可见性等优点,横向流动免疫分析(LFA)已广泛应用于食品安全领域。但是,需要解决 LFA 的低灵敏度问题。纳米酶由于其优异且丰富的类酶特性,在生物传感器中具有惊人的应用潜力。在这项研究中,通过一步法合成的 Au@Pt 纳米酶与辣根过氧化物酶 (HRP) 相比,与 TMB/H2O2 具有更高的亲和力和更高的催化效率。用于检测链霉素 (STR),一种典型的氨基糖苷类抗生素,一种基于 Au@Pt 作为视觉标签的新型 LFA 和基于 Au@Pt 酶样活性的增强型 LFA,通过添加显色底物 3-氨基-9-乙基-咔唑 (AEC) 被建立并与基于 AuNP 的常规 LFA 进行比较。与 8 ng 相比,基于 Au@Pt 作为视觉标签的 LFA 的定性检测限 (LOD) 为 1 ng mL-1,基于 Au@Pt 活性的增强 LFA 的定性检测限 (LOD) 为 0.1 ng mL-1 mL-1 用于基于 AuNP 的 LFA。此外,基于Au@Pt纳米酶的新型LFA成功评估了镇江市牛奶样品中链霉素的含量。这些结果表明,基于纳米酶的 LFA 是一种有前途且有效的食品安全工具。
更新日期:2020-08-01
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