Cell sheet engineering has attracted great attention because thin layers of tissue can be easily transplanted to defect sites. Wound-dressing materials are required to support fast re-epithelization, both with keratinocytes and fibroblasts, to enhance the prognosis and therapeutic outcomes. We prepared self-assembled cell sheets composed of adipocyte-derived stem cells (ADSCs) and surface-engineered nanofibrils (NFs). NFs were surface-engineered with multilayers of gelatin so that the cell sheets could spontaneously assemble within 3 days in cell culture plates. Dorsal wounds transplanted with the cell sheets exhibited higher wound-healing rates when a high concentration of gelatin was immobilized on the surfaces of the NFs. Histochemical staining revealed that those with gelatin-immobilized NFs showed a higher expression of cytokeratin and collagen in the re-epithelized epidermis. Keratinocytic differentiation of the epidermis was molecularly evidenced by the higher expression of keratinocyte-specific genes.

中文翻译：

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由间充质干细胞和明胶纳米纤维组成的自组装细胞片，用于治疗全层伤口。

细胞片工程已经引起了极大的关注，因为薄薄的组织可以很容易地移植到缺损部位。需要伤口敷料来支持角质形成细胞和成纤维细胞的快速再上皮化，以增强预后和治疗效果。我们准备了由脂肪细胞衍生的干细胞（ADSCs）和表面工程纳米纤维（NFs）组成的自组装细胞片。NF是用多层明胶进行表面工程处理的，因此细胞片可以在3天之内在细胞培养板上自发组装。当将高浓度的明胶固定在NFs的表面上时，移植有细胞片的背侧伤口表现出较高的伤口愈合率。组织化学染色显示，那些明胶固定的NFs在重新上皮的表皮中显示出较高的细胞角蛋白和胶原蛋白表达。角质形成细胞特异性基因的更高表达在分子上证明了表皮的角质形成细胞分化。