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Compositional analysis of transgenic Bt-chickpea resistant to Helicoverpa armigera.
GM Crops & Food ( IF 3.9 ) Pub Date : 2020-06-27 , DOI: 10.1080/21645698.2020.1782147
Rubi Gupta 1, 2 , Ananta Madhab Baruah 3 , Sumita Acharjee 1, 2 , Bidyut Kumar Sarmah 1
Affiliation  

Transgenic chickpeas expressing high levels of a truncated version of the cry1Ac (trcry1Ac) gene conferred complete protection to Helicoverpa armigera in the greenhouse. Homozygous progeny of two lines, Cry1Ac.1 and Cry1Ac.2, had similar growth pattern and other morphological characteristics, including seed yield, compared to the non-transgenic counterpart; therefore, seed compositional analysis was carried out. These selected homozygous chickpea lines were selfed for ten generations along with the non-transgenic parent under contained conditions. A comparative seed composition assessment, seed storage proteins profiling, and in vitro protein digestibility were performed to confirm that these lines do not have significant alterations in seed composition compared to the parent. Our analyses showed no significant difference in primary nutritional composition between transgenic and non-transgenic chickpeas. In addition, the seed storage protein profile also showed no variation between the transgenic chickpea lines. Seed protein digestibility assays using simulated gastric fluid revealed a similar rate of digestion of proteins from the transgenic trcry1Ac lines compared to the non-transgenic line. Thus, our data suggest no unintended changes in the seed composition of transgenic chickpea expressing a trcry1Ac gene.



中文翻译:

抗棉铃虫转基因Bt-鹰嘴豆的成分分析。

表达高水平的cry1Ac(tr cry1Ac)基因的截短版本的转基因鹰嘴豆为温室中的棉铃虫提供了完全保护。与非转基因对应物相比,Cry1Ac.1和Cry1Ac.2这两个品系的纯合子代具有相似的生长模式和其他形态特征,包括种子产量。因此,进行了种子组成分析。将这些选择的纯合鹰嘴豆品系与非转基因亲本在封闭条件下自交十代。比较种子组成评估,种子贮藏蛋白分析和体外进行蛋白质消化率的研究以证实与亲本相比,这些品系在种子组成上没有显着改变。我们的分析表明,转基因鹰嘴豆和非转基因鹰嘴豆的主要营养成分没有显着差异。此外,种子贮藏蛋白谱也显示转基因鹰嘴豆品系之间没有变化。使用模拟胃液进行的种子蛋白消化率测定显示,与非转基因株系相比,来自转基因tr cry1Ac株系的蛋白质消化速率相似。因此,我们的数据表明表达tr cry1Ac基因的转基因鹰嘴豆种子组成中没有意外变化。

更新日期:2020-07-24
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