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Methanohalophilus profundi sp. nov., a methylotrophic halophilic piezophilic methanogen isolated from a deep hypersaline anoxic basin.
Systematic and Applied Microbiology ( IF 3.4 ) Pub Date : 2020-06-27 , DOI: 10.1016/j.syapm.2020.126107
Stéphane L'Haridon 1 , Hani Haroun 1 , Erwan Corre 2 , Erwan Roussel 1 , Morgane Chalopin 1 , Patricia Pignet 1 , Charlotte Balière 1 , Violetta la Cono 3 , Mohamed Jebbar 1 , Michail Yakimov 3 , Laurent Toffin 1
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A novel anaerobic methylotrophic halophilic methanogen strain SLHTYROT was isolated from a deep hypersaline anoxic basin called “Tyro” located in the Eastern Mediterranean Sea. Cells of SLHTYROT were motile cocci. The strain SLHTYROT grew between 12 and 37 °C (optimum 30 °C), at pH between 6.5 and 8.2 (optimum pH 7.5) and salinity from 45 to 240 g L−1 NaCl (optimum 135 g L−1). Strain SLHTYROT was methylotrophic methanogen able to use methylated compounds (trimethylamine, dimethylamine, monomethylamine and methanol). Strain SLHTYROT was able to grow at in situ hydrostatic pressure and temperature conditions (35 MPa, 14 °C). Phylogenetic analysis based on 16S rRNA gene and mcrA gene sequences indicated that strain SLHTYROT was affiliated to genus Methanohalophilus within the order Methanosarcinales. It shared >99.16% of the 16S rRNA gene sequence similarity with strains of other Methanohalophilus species. Based on ANIb, AAI and dDDH measurements, and the physiological properties of the novel isolate, we propose that strain SLHTYROT should be classified as a representative of a novel species, for which the name Methanohalophilus profundi sp. nov. is proposed; the type strain is SLHTYROT (=DSM 108854 = JCM 32768 = UBOCC-M-3308).



中文翻译:

甲基嗜盐菌 nov。,从深部高盐缺氧盆地中分离出的一种甲基营养性嗜盐性嗜甲烷性产甲烷菌。

一种新的甲基营养厌氧产甲烷菌嗜盐应变SLHTYRO Ť从称为“TYRO”位于东地中海深超盐度缺氧盆隔离。SLHTYRO T的细胞是运动球菌。SLHTYRO T菌株在12至37°C(最佳30°C)之间生长,pH在6.5至8.2(最佳pH 7.5)之间,盐度为45至240 g L -1 NaCl(最佳135 g L -1)。菌株SLHTYRO T是甲基营养型产甲烷菌,能够使用甲基化的化合物(三甲胺,二甲胺,一甲胺和甲醇)。SLHTYRO T菌株能够原位生长静水压力和温度条件(35 MPa,14°C)。基于16S rRNA基因和系统发育分析MCRA基因序列表明菌株SLHTYRO Ť是附属于属Methanohalophilus顺序内Methanosarcinales。它与其他Methanohalophilus菌株共享16S rRNA基因序列相似性> 99.16%。基于ANIb,AAI和dDDH的测量值,以及新分离株的生理特性,我们建议将SLHTYRO T菌株归类为新物种的代表,其名称为Methanohalophilus profundi sp。十一月 被提议 类型菌株为SLHTYRO T (= DSM 108854 = JCM 32768 = UBOCC-M-3308)。

更新日期:2020-06-27
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