Abstract The objective of this study was to investigate the counteracting effects of glycerol or rumen-protected choline chloride supplementation on rumen fermentation and the profile changes in blood metabolites in pregnant ewes suffering from negative energy balance. Eighteen pregnant Hu sheep with a gestation period of 100 days were used in the present study. Feed restriction was started from 100 days of gestation and lasted for 9 days. During the period of feed restriction, these sheep were randomly assigned to three groups: the control group (CON, n = 6), which received no supplementation, the glycerol group (GLY, n = 6), which received 40 mL of glycerol per day, and the rumen-protected choline chloride group (RPC, n = 6), which received 10 g of rumen-protected choline chloride per day lasted 9 days. All supplements were mixed with feed. The results showed that, compared to CON, glycerol supplementation decreased the proportion of acetate (P d -Glucose have a positive correlation with propionate (r = 0.48, P = 0.02), while have a negative correlation with BHBA (r = −0.77, P

中文翻译：

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甘油或瘤胃保护氯化胆碱对能量负平衡妊娠母羊瘤胃发酵及血液代谢组的影响

摘要 本研究的目的是研究甘油或瘤胃保护的氯化胆碱补充剂对能量负平衡的妊娠母羊瘤胃发酵和血液代谢物谱变化的抵消作用。本研究使用了 18 只怀孕期为 100 天的胡羊。限食从妊娠 100 天开始，持续 9 天。在限饲期间，这些羊被随机分配到三组：对照组（CON，n = 6），不接受补充；甘油组（GLY，n = 6），每组接受 40 mL 甘油。天，瘤胃保护氯化胆碱组（RPC，n = 6）每天接受 10 g 瘤胃保护氯化胆碱，持续 9 天。所有补充剂均与饲料混合。