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Strategies in a metallophyte species to cope with manganese excess.
Environmental Geochemistry and Health ( IF 4.2 ) Pub Date : 2020-06-26 , DOI: 10.1007/s10653-020-00625-z
Sabina Rossini-Oliva 1 , Maria Manuela Abreu 2 , Eduardo Oscar Leidi 3
Affiliation  

The effect of exposure to high Mn concentration was studied in a metallophyte species, Erica andevalensis, using hydroponic cultures with a range of Mn concentrations (0.06, 100, 300, 500, and 700 mg L−1). At harvest, biomass production, element uptake, and biochemical indicators of metal stress (leaf pigments, organic acids, amino acids, phenols, and activities of catalase, peroxidase, superoxide dismutase) were determined in leaves and roots. Increasing Mn concentrations led to a decrease in biomass accumulation, and tip leaves chlorosis was the only toxicity symptom detected. In a similar way, photosynthetic pigments (chlorophylls a and b, and carotenoids) were affected by high Mn levels. Among organic acids, malate and oxalate contents in roots showed a significant increase at the highest Mn concentration, while in leaves, Mn led to an increasing trend in citrate and malate contents. An increase of Mn also induced an increase in superoxide dismutase activity in roots and catalase activity in leaves. As well, significant changes in free amino acids were induced by Mn concentrations higher than 300 mg L−1, especially in roots. No significant changes in phenolic compounds were observed in the leaves, but root phenolics were significantly increased by increasing Mn concentrations in treatments. When Fe supply was increased 10 and 20 times (7–14 mg Fe L−1 as Fe-EDDHA) in the nutrient solutions at the highest Mn concentration (700 mg Mn L−1), it led to significant increases in photosynthetic pigments and biomass accumulation. Manganese was mostly accumulated in the roots, and the species was essentially a Mn excluder. However, considering the high leaf Mn concentration recorded without toxicity symptoms, E. andevalensis might be rated as a Mn-tolerant species.



中文翻译:

金属植物种应对过量锰的策略。

在金属植物种类Erica andevalensis中,使用了一系列Mn浓度(0.06、100、300、500和700 mg L -1)的水培培养方法,研究了暴露于高Mn浓度的影响。在收获时,确定了叶和根中金属胁迫的生物量生产,元素吸收和生化指标(叶色素,有机酸,氨基酸,苯酚以及过氧化氢酶,过氧化物酶,超氧化物歧化酶的活性)。锰浓度的增加导致生物量积累的减少,而尖叶萎黄是唯一检测到的毒性症状。以类似的方式,光合色素(叶绿素ab,和类胡萝卜素)受到高锰水平的影响。在有机酸中,最高的Mn浓度下,根中的苹果酸和草酸含量显着增加,而在叶片中,Mn导致柠檬酸和苹果酸的含量呈增加趋势。锰的增加还诱导根部的超氧化物歧化酶活性和叶片中的过氧化氢酶活性增加。同样,高于300 mg L -1的Mn浓度会引起游离氨基酸的显着变化,尤其是在根部。在叶片中未观察到酚类化合物的显着变化,但通过增加处理中Mn的浓度,根部酚类物质显着增加。当铁的供应增加10倍和20倍时(7-14 mg Fe L -1在最高锰浓度(700 mg Mn L -1)的营养液中添加铁(ED-EDDHA),导致光合色素和生物量积累显着增加。锰大部分积累在根中,而该种实质上是锰的排阻剂。但是,考虑到记录的高叶片Mn浓度没有毒性症状,因此E. andevalensis可以被认为是Mn耐性物种。

更新日期:2020-06-27
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