To provide new choices and to replace the complicated steps of synthesizing modified electrodes, this research reports the systematic investigation of the effects of different supporting electrolytes on the electrochemical behavior of vitamin B₂ (VB₂), vitamin B₆ (VB₆), and vitamin C (VC) using a common screen-printed carbon electrode (SPCE) to achieve simultaneous detection. The roles of various well-known supporting electrolytes, including Britton–Robinson buffer solution (BRBS), acetate buffer solution (ABS), and phosphate buffer solution (PBS), in the electrochemical oxidation of VB₂, VB₆, and VC were carefully evaluated by square wave voltammetry (SWV). From a successive investigation based on the ionic conductivity of the species contained in the supporting electrolytes, PBS at pH 3.5 was selected as a suitable supporting electrolyte for the simultaneous detection of VB₂, VB₆, and VC. Under the optimized conditions, the simultaneous detection of VB₂, VB₆, and VC provided satisfactory sensitivity, selectivity, and reproducibility even when using a common electrode. To demonstrate the practicality and reliability of the proposed analytical procedure, the simultaneous detection of VB₂, VB₆, and VC was performed in mixed vegetable and fruit juice samples and urine samples by SWV, and the results were compared with those obtained by chromatographic detection. Therefore, this method was found to be simple and may be a new alternative for the simultaneous determination of VB₂, VB₆, and VC in practical applications.

中文翻译：

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支持电解质对使用无修饰丝网印刷碳电极同时测定维生素B2，维生素B6和维生素C的影响

为了提供新的选择并取代合成修饰电极的复杂步骤，本研究报告了系统研究不同支持电解质对维生素B ₂（VB ₂），维生素B ₆（VB ₆）和维生素C（VC）使用普通的丝网印刷碳电极（SPCE）来实现同时检测。在VB ₂，VB ₆的电化学氧化中，包括Britton-Robinson缓冲溶液（BRBS），乙酸盐缓冲溶液（ABS）和磷酸盐缓冲溶液（PBS）在内的各种众所周知的辅助电解质的作用和VC是通过方波伏安法（SWV）仔细评估的。从基于支持电解质中所含物质的离子电导率的连续研究中，选择pH为3.5的PBS作为同时检测VB ₂，VB ₆和VC的合适支持电解质。在优化的条件下，即使使用公共电极，同时检测VB ₂，VB ₆和VC也可以提供令人满意的灵敏度，选择性和可重复性。为了证明所提出的分析程序的实用性和可靠性，同时检测VB ₂，VB ₆，并通过SWV在蔬菜和果汁混合样品和尿液样品中进行VC，并将结果与​​色谱检测获得的结果进行比较。因此，发现该方法很简单，并且可能是在实际应用中同时测定VB ₂，VB ₆和VC的新替代方法。