Consumption of non-nutritive sweeteners (NNS) has been consistently associated with obesity and cardiometabolic disease in epidemiologic studies. Herein, we investigated effects of sucralose, a widely used NNS, at a cellular level. We wanted to investigate effect of sucralose on reactive oxygen species accumulation and adipogenesis in a human adipocyte tissue-derived mesenchymal stromal cells (MSCs) in a controlled fashion. In vitro experiments were conducted on commercially available MSCs obtained from human adipose tissue. hMSCs were exposed with sucralose at 0.2 mM (a concentration which could plausibly be observed in the circulatory system of high NNS consumers) up to 1.0 mM (supra-physiologic concentration) in the presence of both normal and high glucose media to detect a dose response based on the outcome measures. Reactive oxygen species (ROS) were detected using Mitosox Red staining and further analyzed by ImageJ and gene expression analysis. Effect of sucralose on adipogenic differentiation was observed in different concentrations of sucralose followed by gene expression analysis and Oil Red O staining. Increased ROS accumulation was observed within 72 h of exposure. Increased adipogenesis was also noted when exposed to higher dose of sucralose. Sucralose promotes ROS accumulation and adipogenesis in human adipose tissue derived mesenchymal stromal cells.

中文翻译：

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三氯蔗糖促进间充质基质细胞中活性氧（ROS）的积累和脂肪形成。

在流行病学研究中，食用非营养性甜味剂（NNS）一直与肥胖和心脏代谢疾病相关。在本文中，我们研究了三氯蔗糖（一种广泛使用的NNS）在细胞水平上的作用。我们想要研究三氯半乳蔗糖对人类脂肪细胞组织间充质基质细胞（MSCs）中活性氧的积累和脂肪形成的影响。在从人脂肪组织获得的可商购的MSC上进行了体外实验。在正常和高葡萄糖培养基均存在的情况下，将hMSCs暴露于高达1.0 mM（超生理浓度）的0.2 mM三氯蔗糖（在高NNS消费者的循环系统中可能观察到的浓度）以检测剂量反应基于结果度量。使用Mitosox Red染色检测活性氧（ROS），并通过ImageJ和基因表达分析进一步分析。在不同浓度的三氯半乳蔗糖中观察到三氯半乳蔗糖对成脂分化的影响，随后进行基因表达分析和油红O染色。暴露后72小时内观察到ROS积累增加。当暴露于更高剂量的三氯蔗糖时，脂肪形成也增加。三氯蔗糖促进人脂肪组织来源的间充质基质细胞中ROS的积累和脂肪生成。暴露后72小时内观察到ROS积累增加。当暴露于更高剂量的三氯蔗糖时，脂肪形成也增加。三氯蔗糖促进人脂肪组织来源的间充质基质细胞中ROS的积累和脂肪生成。暴露后72小时内观察到ROS积累增加。当暴露于更高剂量的三氯蔗糖时，脂肪形成也增加。三氯蔗糖促进人脂肪组织来源的间充质基质细胞中ROS的积累和脂肪生成。