It was demonstrated in our previous research that trypsin scavenges superoxide anions. In this study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus. Trypsin significantly delayed the weight loss and decreased the levels of ROS and membrane lipid peroxidation. Transcriptome profiles of H. undatus treated with trypsin revealed the pathways and regulatory mechanisms of ROS genes that were up- or downregulated following trypsin treatment by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The current results showed that through the regulation of the expression of hub redox enzymes, especially thioredoxin-related proteins, trypsin can maintain low levels of endogenous active oxygen species, reduce malondialdehyde content and delay fruit aging. In addition, the results of protein-protein interaction networks suggested that the downregulated NAD(P) H and lignin pathways might be the key regulatory mechanisms governed by trypsin. Trypsin significantly prolonged the storage life of H. undatus through regulatory on the endogenous ROS metabolism. As a new biopreservative, trypsin is highly efficient, safe and economical. Therefore, trypsin possesses technical feasibility for the quality control of fruit storage.

中文翻译：

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转录组学分析揭示了在储存过程中通过新颖的超氧化物清除剂胰蛋白酶处理了Hylocereus undatus中与ROS代谢相关的中心基因和子网络。

我们以前的研究表明，胰蛋白酶可清除超氧阴离子。在这项研究中，评估了通过胰蛋白酶提高储存质量的机制。胰蛋白酶显着延迟了体重减轻，并降低了ROS和膜脂质过氧化的水平。用胰蛋白酶处理过的H. undatus的转录组图谱揭示了通过基因本体论（GO）和《京都议定书》的基因和基因组途径（KEGG）富集分析，胰蛋白酶处理后ROS基因的上调或下调的途径和调控机制。目前的结果表明，通过调节轮毂氧化还原酶，特别是硫氧还蛋白相关蛋白的表达，胰蛋白酶可以维持低水平的内源性活性氧，降低丙二醛含量并延缓果实衰老。此外，蛋白质-蛋白质相互作用网络的结果表明，NAD（P）H和木质素途径的下调可能是胰蛋白酶控制的关键调控机制。胰蛋白酶通过调节内源性ROS代谢显着延长了H. undatus的储存寿命。作为一种新型的生物防腐剂，胰蛋白酶是高效，安全和经济的。因此，胰蛋白酶具有控制水果贮藏质量的技术可行性。