Sea cucumber promotes multifaceted health benefits. However, the mechanisms of sea cucumber peptides (Scp) regulating the antifatigue capacity is still unknown. The present study is aimed at further elucidating the effects and mechanisms of Scp on the antifatigue capacity of mice. At first, C57BL/6J mice were assigned into four groups named Con, L-Scp, M-Scp, and H-Scp and received diets containing Scp (0%, 0.15%, 0.3%, and 0.5%, respectively) for continuous 30 days. On the 21th day, a fore grip test was conducted on mice. On the 25th day, a rotating rod test was conducted on mice. On the 30th day, the quantities of glycogen and mitochondrial DNA (mtDNA) were determined in 8 random mice and another 8 mice were forced to swim for 1 hour before slaughter for detecting biochemical indicators. It was observed that the Scp groups significantly prolonged the running time in rotarod, increased forelimb grip strength, improved lactic acid (LD) and urea nitrogen (BUN) levels in the serum, decreased lactic dehydrogenase (LDH) and glutamic oxalacetic transaminase (GOT) activities in the serum, increased blood glucose (BG) and glycogen (GN) levels in the liver and skeletal muscle after swimming, increased the activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in the skeletal muscle and heart, and improved antioxidant capacity. Furthermore, Scp treatment significantly elevated the mRNA and protein relative levels of power-sensitive factors, lipid catabolism, and mitochondrial biogenesis and significantly upregulated mRNA levels of gluconeogenesis. Besides, mtDNA before the swimming test was increased in the three Scp groups. These results show that Scp treatment has antifatigue capacity. Furthermore, these results suggest that improved energy regulation and antioxidant capacity may be the result of improved mitochondrial function.

中文翻译：

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海参肽改善了小鼠的线粒体能力：增强运动中糖原异生和脂肪代谢的潜在机制，以改善抗疲劳特性。

海参可促进多方面的健康益处。然而，海参肽（Scp）调节抗疲劳能力的机制仍是未知的。本研究旨在进一步阐明Scp对小鼠抗疲劳能力的影响及其机制。首先，将C57BL / 6J小鼠分为四组，分别称为Con，L-Scp，M-Scp和H-Scp，并接受含Scp（分别为0％，0.15％，0.3％和0.5％）的饮食以连续30天。在第21天，对小鼠进行前握力测试。在第25天，对小鼠进行了旋转棒试验。在第30天，测定了8只随机小鼠的糖原和线粒体DNA（mtDNA）的量，并在屠宰前将另外8只小鼠游泳1小时以检测生化指标。骨骼肌和心脏中的⁺ -K ⁺ -ATPase和Ca ²⁺ -Mg ²⁺ -ATPase，并提高了抗氧化能力。此外，Scp处理显着提高了功率敏感因子，脂质分解代谢和线粒体生物发生的mRNA和蛋白质相对水平，并显着上调了糖异生的mRNA水平。此外，在三个Scp组中，游泳测试前的mtDNA有所增加。这些结果表明，Scp治疗具有抗疲劳能力。此外，这些结果表明改善的能量调节和抗氧化能力可能是改善的线粒体功能的结果。