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Polyphasic taxonomic analysis of Sinimarinibacterium arenosum sp. nov., a halophilic bacterium isolated from marine sediment.
FEMS Microbiology Letters ( IF 2.1 ) Pub Date : 2020-06-26 , DOI: 10.1093/femsle/fnaa105
Jong-Hwa Kim 1 , Jihye Baek 1 , Jung-Hoon Yoon 2 , Ampaitip Sukhoom 3 , Wonyong Kim 1
Affiliation  

A Gram stain-negative, aerobic, motile, rod-shaped bacterial strain, designated CAU 1509T, was isolated from marine sediment, and its taxonomic position was determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1509T formed a distinct lineage within the genus Sinimarinibacterium with the highest 16S rRNA gene sequence similarity to Sinimarinibacterium flocculans NH6–24T (97.0%). Similar to another member of Sinimarinibacterium, ubiquinone-8 (Q-8) was the predominant quinone, whereas C16:0, summed feature 3 (C16:1ω7c/ω6c) and summed feature 8 (C18:1ω7c/ω6c) were the major cellular fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, four unidentified glycolipids and three unidentified aminolipids. Strain CAU 1509T has a genome of 4.54 Mb, including 4251 coding sequences, 6 rRNAs and 50 tRNAs, with a genomic DNA G+C content of 63.2 mol%. Based on its phenotypic, chemotaxonomic and phylogenetic properties, strain CAU 1509T should be classified as a novel species of the genus Sinimarinibacterium, for which the name Sinimarinibacterium arenosum sp. nov. is proposed. The type strain is CAU 1509T (= KCTC 72000T = NBRC 113698T).

中文翻译:

沙门氏菌的多相分类学分析。nov。,一种从海洋沉积物中分离出来的嗜盐细菌。

从海洋沉积物中分离出革兰氏阴性,好氧,能运动的杆状细菌菌株,命名为CAU 1509 T,并使用多相方法确定了其分类学位置。基于16S rRNA基因序列的系统进化分析表明,菌株CAU 1509 TSinimarinibacter属内形成了一个独特的谱系,与絮状Sinimarinibacterium flocculans NH6-24 T的最高16S rRNA基因序列相似性(97.0%)。类似的另一个成员Sinimarinibacterium,泛醌-8(Q-8)是主要的醌,而C则16:0,总结特征3(C 16:1 ω 7 Ç /ω 6 Ç)和求和特征8(C 18:1 ω 7 ç / ω 6 Ç)是主要细胞脂肪酸。主要的极性脂质是二磷脂酰甘油,磷脂酰甘油,磷脂酰乙醇胺,未鉴定的磷脂,四个未鉴定的糖脂和三个未鉴定的氨基脂。菌株CAU 1509 T的基因组为4.54 Mb,包括4251个编码序列,6个rRNA和50个tRNA,基因组DNA G + C含量为63.2 mol%。基于其表型,化学分类和系统发育性能,应变CAU 1509 Ť应列为属的一个新种Sinimarinibacterium,为此,名称Sinimarinibacterium arenosum SP。十一月 被提议。类型应变为CAU 1509 T(= KCTC 72000 T  = NBRC 113698 T)。
更新日期:2020-07-09
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