Sealants are useful as agents that can prevent the leakage of gas or nonclotting fluids from damaged tissues and of blood from the vascular system following injury or repair. Various formulations for sealants have been developed and applied clinically, but problems still remain in terms of biocompatibility issues, long crosslinking times and low adhesive properties. Herein, to address these issues, we report a methacrylated silk fibroin sealant (Sil-MAS) with rapidly crosslinkable, highly adhesive and biocompatible properties and demonstrate its versatility as a medical glue. The excellent physical properties of Sil-MAS are revealed via in vitro mechanical tests and ex vivo aorta pressure tests. In addition, in in vivo biological tests on the skin, liver, and blood vessels of rats, Sil-MAS showed a superb hemostatic and adhesive ability, with high biocompatibility. Specifically, Sil-MAS strongly contributed to faster wound healing than commercially available materials. Furthermore, we showed a successful proof of concept that Sil-MAS could serve as an ideal photocuring laparoscopic medical glue in a laceration rabbit model of liver and stomach serosa using a homemade endoscopic device. These findings on the applicability of rapidly photocurable silk fibroin indicate that Sil-MAS is a suitable material to supplant existing sealants, adhesives, or hemostatic agents.

密封剂可用作在受伤或修复后可防止气体或非凝结液从受损组织泄漏以及血管系统血液泄漏的试剂。已经开发出多种密封剂配方并在临床上应用，但是在生物相容性问题，长交联时间和低粘合性能方面仍然存在问题。在本文中，为了解决这些问题，我们报告了一种甲基丙烯酸酯化的丝素蛋白密封剂（Sil-MAS），具有快速可交联，高度粘合和生物相容性的特性，并证明了其作为医用胶水的多功能性。Sil-MAS的优异物理性能通过体外机械测试和离体主动脉压力测试得以揭示。此外，在对大鼠的皮肤，肝脏和血管进行的体内生物学测试中，Sil-MAS显示出极好的止血和粘附能力，具有高生物相容性。具体而言，与市售材料相比，Sil-MAS对加快伤口愈合有很大贡献。此外，我们展示了一个成功的概念证明，即使用自制的内窥镜设备，Sil-MAS可以作为理想的光固化腹腔镜医用胶在肝胃浆膜撕裂兔模型中使用。这些关于可快速光固化的丝素蛋白的适用性的发现表明，Sil-MAS是替代现有密封剂，粘合剂或止血剂的合适材料。我们展示了一个成功的概念证明，即使用自制的内窥镜设备，Sil-MAS可以作为理想的光固化腹腔镜医用胶水用于肝脏和胃浆膜撕裂兔模型。这些关于可快速光固化的丝素蛋白的适用性的发现表明，Sil-MAS是替代现有密封胶，粘合剂或止血剂的合适材料。我们展示了一个成功的概念证明，即使用自制的内窥镜设备，Sil-MAS可以作为理想的光固化腹腔镜医用胶水用于肝脏和胃浆膜撕裂兔模型。这些关于可快速光固化的丝素蛋白的适用性的发现表明，Sil-MAS是替代现有密封剂，粘合剂或止血剂的合适材料。