Circular RNAs (circRNAs) correlate with cancer cell phenotypes. Particularly, circRNAs mediate the cancer process as microRNAs (miRNAs) sponges. This study was to ascertain the roles of hsa_circ_0009910 in phenotypic aspects of ovarian cancer cells. Mantel-Cox test was performed to analyze the correlation between hsa_circ_0009910 and survival outcomes of ovarian cancer. Minigene reporter was constructed and small interfering-RNA was designed for constructing hsa_circ_0009910-dysregulated and miR-145-upregulated cells identified by qRT-PCR. Proliferative and motile activities were monitored by CCK and Transwell. Western blot was applied for quantification of cyclin D1, CDK4, CDK6, MMP-2, MMP-9, IκBα, p65, Notch1, Hes1, and Hes5. miRNAs targets were predicted using a bioinformatics tool and confirmed using qRT-PCR and Dual-Luciferase reporter assay. Hsa_circ_0009910 was correlated with the poor prognosis of ovarian cancer patients. The ovarian cancer cell phenotypes were promoted by hsa_circ_0009910 while repressed by silencing hsa_circ_0009910. Hsa_circ_0009910 silence was responsible for the upregulation of the predicted miRNAs targets. Thereinto, miR-145 was confirmed as a miRNA target and negatively regulated by hsa_circ_0009910. miR-145 nullified the biological function of hsa_circ_0009910 in the proliferative and motile phenotypes, and the active status of NF-κB and Notch. Hsa_circ_0009910, representing unfavorable prognosis, induced proliferative and motile phenotypes by suppressing miR-145 in ovarian cancer cells.

环状RNA（circRNA）与癌细胞表型相关。特别是，circRNA作为microRNA（miRNA）海绵介导了癌症过程。这项研究是要确定hsa_circ_0009910在卵巢癌细胞表型方面的作用。进行Mantel-Cox检验以分析hsa_circ_0009910与卵巢癌生存结果之间的相关性。构建了小基因报告基因，并设计了小干扰RNA，用于构建经qRT-PCR鉴定的hsa_circ_0009910失调和miR-145上调的细胞。通过CCK和Transwell监测增殖和运动活动。Western印迹用于定量细胞周期蛋白D1，CDK4，CDK6，MMP-2，MMP-9，IκBα，p65，Notch1，Hes1和Hes5。使用生物信息学工具预测了miRNA靶标，并使用qRT-PCR和双荧光素酶报告基因测定法确认了miRNA靶标。Hsa_circ_0009910与卵巢癌患者预后差有关。hsa_circ_0009910促进卵巢癌细胞表型，而沉默hsa_circ_0009910则抑制卵巢癌细胞表型。Hsa_circ_0009910沉默是导致预测的miRNA靶标上调的原因。其中，miR-145被确认为miRNA靶标，并受hsa_circ_0009910负调控。miR-145使hsa_circ_0009910在增殖和运动型表型以及NF-κB和Notch的活跃状态中的生物学功能无效。表示不良预后的Hsa_circ_0009910通过抑制卵巢癌细胞中的miR-145诱导增殖性和活动性表型。hsa_circ_0009910促进卵巢癌细胞表型，而沉默hsa_circ_0009910则抑制卵巢癌细胞表型。Hsa_circ_0009910沉默是导致预测的miRNA靶标上调的原因。其中，miR-145被确认为miRNA靶标，并受hsa_circ_0009910负调控。miR-145使hsa_circ_0009910在增殖和运动型表型以及NF-κB和Notch的活跃状态中的生物学功能无效。表示不良预后的Hsa_circ_0009910通过抑制卵巢癌细胞中的miR-145诱导增殖性和活动性表型。hsa_circ_0009910促进卵巢癌细胞表型，而沉默hsa_circ_0009910则抑制卵巢癌细胞表型。Hsa_circ_0009910沉默是导致预测的miRNA靶标上调的原因。其中，miR-145被确认为miRNA靶标，并受hsa_circ_0009910负调控。miR-145使hsa_circ_0009910在增殖和运动型表型以及NF-κB和Notch的活跃状态中的生物学功能无效。表示不良预后的Hsa_circ_0009910通过抑制卵巢癌细胞中的miR-145诱导增殖性和活动性表型。miR-145使hsa_circ_0009910在增殖和运动型表型以及NF-κB和Notch的活跃状态中的生物学功能无效。表示不良预后的Hsa_circ_0009910通过抑制卵巢癌细胞中的miR-145诱导增殖性和活动性表型。miR-145使hsa_circ_0009910在增殖和运动型表型以及NF-κB和Notch的活跃状态中的生物学功能无效。表示不良预后的Hsa_circ_0009910通过抑制卵巢癌细胞中的miR-145诱导增殖性和活动性表型。