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Natural rubber biodegradation by Alternaria alternata and Penicillium chrysogenum isolates
Bioremediation Journal ( IF 2 ) Pub Date : 2020-06-26 , DOI: 10.1080/10889868.2020.1777931
Chiara Mollea 1 , Francesca Bosco 1
Affiliation  

Abstract In the present work, the biodegradation of natural rubber by means of three filamentous fungi, two Alternaria alternata strains (ANR and BNR) and Penicillium chrysogenum, is reported. The biodegradation process has been optimized in Erlenmeyer flasks and then scaled-up in Fernbach flasks. The biodegradation was performed in stationary liquid cultures with biomass immobilized on ceramic Berl saddles. In the presence of NR as the only carbon source, the maximum value of enzyme activity (0.242 IU/L) was reached at 41 days of incubation and at the end of incubation time, 65 days, a 2% weight loss of NR was detected. In Fernbach flasks, the maximum peroxidase activity value was doubled (0.500 IU/L) and reached earlier, after 6 days. In the same incubation time, a better biodegradation performance (4.3%) was obtained. Moreover, the application of a double biodegradation step allowed to obtain higher biodegradation percentage, 6.8%. Among the different tested microorganisms, A. alternata BNR showed the best biodegradation potentiality in terms of enzyme production and NR degradation. On the basis of the results obtained in liquid cultures, A. alternata BNR is currently applied in a lab-scale microcosm study which has to be considered the preliminary step of an in-field biodegradation process.

中文翻译:

链格孢和黄青霉分离株对天然橡胶的生物降解

摘要 在目前的工作中,报道了通过三种丝状真菌、两种链格孢菌菌株(ANR 和 BNR)和Penicillium chrysogenum 生物降解天然橡胶。生物降解过程已在 Erlenmeyer 烧瓶中优化,然后在 Fernbach 烧瓶中放大。生物降解在固定液体培养物中进行,生物质固定在陶瓷 Berl 鞍座上。在 NR 作为唯一碳源存在的情况下,酶活性的最大值 (0.242 IU/L) 在孵育 41 天时达到,并且在孵育时间结束 65 天时,检测到 NR 的 2% 重量损失. 在 Fernbach 烧瓶中,最大过氧化物酶活性值加倍 (0.500 IU/L) 并在 6 天后提前达到。在相同的孵育时间内,获得了更好的生物降解性能(4.3%)。而且,双生物降解步骤的应用允许获得更高的生物降解百分比,6.8%。在不同的测试微生物中,A. alternata BNR 在酶产生和 NR 降解方面表现出最好的生物降解潜力。根据在液体培养中获得的结果,A. alternata BNR 目前应用于实验室规模的微观研究,必须将其视为现场生物降解过程的初步步骤。
更新日期:2020-06-26
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