Abstract

Recently, scientists have used the metallic nanoparticles especially copper nanoparticles for formulating many new neuroprotective supplements in the field of neurology. Also, we know the role of Nigella sativa L. in increasing the physiological activities of the central nervous system in traditional medicine. In the present study, we decided to prepare and formulate a new neuroprotective supplement (copper nanoparticles in aqueous medium using N. sativa seed aqueous extract) in the in vitro condition. The organometallic chemistry tests such as Fourier Transformed Infrared Spectroscopy (FT‐IR), UV–Visible Spectroscopy (UV-Vis), Field Emission Scanning Electron Microscopy (FE‐SEM), and Transmission Electron Microscopy (TEM) were used for characterizing of copper nanoparticles. In the FT-IR test, the presence of many antioxidant compounds with related bonds caused excellent condition for reducing copper in the copper nanoparticles. In UV-Vis, the clear peak in the wavelength of 569 nm showed the copper nanoparticles formation. Also, in the TEM and FE-SEM images, the copper nanoparticles had the size of 19.5 nm. In the biological part of the current study, methadone significantly (p ≤ 0.01) decreased cell viability, mitochondrial membrane potential, and increased inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation. CuNPs-treated cell cutlers significantly (p ≤ 0.01) increased cell viability and mitochondrial membrane potential, and decreased inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation in the high concentration of methadone-treated adrenal phaeochromocytoma (PC12) cells. For investigating the antioxidant properties of copper nanoparticles, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was used in the presence of butylated hydroxytoluene as the positive control. The copper nanoparticles inhibited half of the DPPH molecules in the concentration of 171 µg/mL. In this study, we concluded that copper nanoparticles biosynthesized by N. sativa L. seed suppressed methadone-induced cell death in PC12 cells.

摘要

最近，科学家已将金属纳米颗粒，特别是铜纳米颗粒用于配制神经病学领域的许多新的神经保护补剂。此外，我们知道黑夜蛾在增加传统医学中中枢神经系统的生理活动中的作用。在本研究中，我们决定在体外制备和配制新的神经保护性补充剂（使用苜蓿种子水提取物的水介质中的铜纳米颗粒）健康）状况。有机金属化学测试（例如傅立叶变换红外光谱（FT-IR），紫外可见光谱（UV-Vis），场发射扫描电子显微镜（FE-SEM）和透射电子显微镜（TEM））用于表征铜纳米粒子。在FT-IR测试中，许多具有相关键的抗氧化剂化合物的存在为还原铜纳米颗粒中的铜提供了极好的条件。在UV-Vis中，在569 nm波长处的清晰峰表明形成了铜纳米颗粒。另外，在TEM和FE-SEM图像中，铜纳米颗粒的尺寸为19.5nm。在本研究中的生物部分，显著美沙酮（p ≤0.01）降低细胞活力，线粒体膜电位，并增加炎症细胞因子浓度，caspase-3活性和DNA片段化。CuNPs处理的细胞刀明显（p ≤0.01）在高浓度美沙酮治疗的肾上腺嗜铬细胞瘤（PC12）细胞中提高了细胞活力和线粒体膜电位，并降低了炎症细胞因子浓度，caspase-3活性和DNA片段化。为了研究铜纳米粒子的抗氧化性能，在丁基化羟基甲苯的存在下，将2,2-二苯基-1-吡啶并肼（DPPH）测试用作阳性对照。铜纳米颗粒以171 µg / mL的浓度抑制了一半的DPPH分子。在这项研究中，我们得出的结论是，由猪笼草种子生物合成的铜纳米颗粒抑制了美沙酮诱导的PC12细胞死亡。