The instrumentation of the in vitro culture system has allowed researchers to learn more about the metabolic and growth behavior of Babesia spp. The various applications for in vitro cultivation of Babesia include obtaining attenuated strains for vaccination or pre-munition, the selection of pure lines with different degrees of virulence, studies on biological cloning, ultrastructure, antigen production for diagnostics, drug sensitivity assessments, and different aspects of parasite biology. Although there are different types of vaccines that have been tested against bovine babesiosis, so far, the only procedure that has offered favorable results in terms of protection and safety has been the use of live attenuated vaccines. In countries, such as Australia, Argentina, Brazil, Uruguay and Israel, this type of vaccine has been produced and used. The alternative to live vaccines other than splenectomized calf-derived biological material, has been the in vitro cultivation of Babesia bovis and B. bigemina. The development of in vitro culture of Babesia spp. strains in a defined medium has been the basis for the initiation of a source of parasites and exoantigens for a variety of studies on the biochemistry and immunology of babesiosis. The use of live immunogens from attenuated strains derived from in vitro culture is highlighted, which has been proposed as an alternative to control bovine babesiosis. In several studies performed in Mexico, this type of immunogen applied to susceptible cattle has shown the induction of protection against the experimental heterologous strain challenge with both, Babesia-infected blood and animal exposure to confrontations on tick vector-infested farms. The combination of transfection technologies and the in vitro culture system as integrated methodologies would eventually give rise to the generation of genetically modified live vaccines. However, a greater challenge faced now by researchers is the large-scale cultivation of Babesia parasites for mass production and vaccine distribution.

仪器仪表的体外培养系统使研究人员能够更多地了解植物的代谢和生长行为巴贝斯虫种。的各种应用体外培养巴贝斯虫包括获得用于疫苗接种或免疫前的减毒株、选择具有不同毒力程度的纯系、生物克隆、超微结构、用于诊断的抗原生产、药物敏感性评估以及寄生虫生物学的不同方面的研究。尽管已经针对牛巴贝斯虫病测试了不同类型的疫苗，但迄今为止，在保护和安全性方面提供良好结果的唯一方法是使用减毒活疫苗。澳大利亚、阿根廷、巴西、乌拉圭、以色列等国家已生产并使用该疫苗。除了摘除脾脏的小牛来源的生物材料之外，活疫苗的替代方案是体外培养牛巴贝斯虫和B.双联。的发展体外的文化巴贝斯虫种。确定培养基中的菌株已成为启动寄生虫和外抗原来源的基础，用于巴贝虫病的生物化学和免疫学的各种研究。使用来自减毒株的活免疫原体外文化被强调，它被提议作为控制牛巴贝斯虫病的替代方法。在墨西哥进行的几项研究中，这种应用于易感牛的免疫原已显示出针对实验性异源菌株攻击的诱导保护作用，巴贝斯虫-受感染的血液和动物在蜱虫媒介出没的农场中接触对抗。转染技术与转染技术的结合体外培养系统作为综合方法最终将产生转基因活疫苗。然而，目前研究人员面临的更大挑战是大规模培育巴贝斯虫用于大规模生产和疫苗分发的寄生虫。