Frontiers in Cellular and Infection Microbiology ( IF 5.7 ) Pub Date : 2020-05-05 , DOI: 10.3389/fcimb.2020.00268 Donghang Li 1 , Ruyuan He 1 , Guoqiang Hou 2 , Wei Ming 2 , Tao Fan 1 , Lei Chen 1 , Lin Zhang 1 , Wenyang Jiang 1 , Wei Wang 1 , Zilong Lu 1 , Haojie Feng 1 , Qing Geng 1
Esophageal microbiota plays important roles in esophageal cancer. Esophagectomy, as the most important therapeutic way, contributes to changes of esophageal microbiome. However, there are few studies examining the esophageal microbiome and the metabolic changes before and after esophagectomy. The present study characterized the esophageal microbiome of 17 patients with esophageal squamous cell carcinoma (ESCC), 11 patients with esophagogastric junction (EGJ) cancer, 15 patients at 9–12 months after radical esophagectomy and 16 healthy controls (HC). 16S ribosomal RNA gene sequencing was used to evaluate the microbiome and predict the metabolic pathways. Our results showed that the microbial diversity was significantly lower in ESCC, EGJ and post-ESCC groups than that in the HC group. The abundance of Fusobacteria was higher (7.01 vs. 1.12%,
中文翻译:
食管微生物群的特征和食管癌相关代谢途径的预测。
食道微生物群在食道癌中起重要作用。食管切除术是最重要的治疗方法,有助于改变食管微生物组。但是,很少有研究检查食管微生物组和食管切除术前后的代谢变化。本研究对17例食管鳞状细胞癌(ESCC),11例食管胃交界处(EGJ)癌,15例在根治性食管切除术后9-12个月的患者和16例健康对照(HC)的食管微生物组进行了表征。使用16S核糖体RNA基因测序来评估微生物组并预测代谢途径。我们的结果表明,ESCC,EGJ和ESCC后组的微生物多样性明显低于HC组。Fusobacteria的丰度更高(7.01比1.12%,