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Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer.
Frontiers in Cellular and Infection Microbiology ( IF 5.7 ) Pub Date : 2020-05-05 , DOI: 10.3389/fcimb.2020.00268
Donghang Li 1 , Ruyuan He 1 , Guoqiang Hou 2 , Wei Ming 2 , Tao Fan 1 , Lei Chen 1 , Lin Zhang 1 , Wenyang Jiang 1 , Wei Wang 1 , Zilong Lu 1 , Haojie Feng 1 , Qing Geng 1
Affiliation  

Esophageal microbiota plays important roles in esophageal cancer. Esophagectomy, as the most important therapeutic way, contributes to changes of esophageal microbiome. However, there are few studies examining the esophageal microbiome and the metabolic changes before and after esophagectomy. The present study characterized the esophageal microbiome of 17 patients with esophageal squamous cell carcinoma (ESCC), 11 patients with esophagogastric junction (EGJ) cancer, 15 patients at 9–12 months after radical esophagectomy and 16 healthy controls (HC). 16S ribosomal RNA gene sequencing was used to evaluate the microbiome and predict the metabolic pathways. Our results showed that the microbial diversity was significantly lower in ESCC, EGJ and post-ESCC groups than that in the HC group. The abundance of Fusobacteria was higher (7.01 vs. 1.12%, P = 0.039) and the abundance of Actinobacteria (1.61 vs. 4.04%) was lower in the ESCC group than that in the HC group. We found significant differences in the abundance of Bacteroidetes (20.45 vs. 9.86%, P = 0.026), Fusobacteria (7.01 vs. 1.66%, P = 0.030) between ESCC and post-ESCC groups. The results of microbial composition analysis and PICRUSt demonstrated significant differences between ESCC and HC groups. The β diversity and PICRUSt suggested that the microbial composition and metabolic pathways were similar to HC group after esophagectomy. The monitoring of the esophagus microbiota may be an essential method to predict the recurrence of tumor.



中文翻译:

食管微生物群的特征和食管癌相关代谢途径的预测。

食道微生物群在食道癌中起重要作用。食管切除术是最重要的治疗方法,有助于改变食管微生物组。但是,很少有研究检查食管微生物组和食管切除术前后的代谢变化。本研究对17例食管鳞状细胞癌(ESCC),11例食管胃交界处(EGJ)癌,15例在根治性食管切除术后9-12个月的患者和16例健康对照(HC)的食管微生物组进行了表征。使用16S核糖体RNA基因测序来评估微生物组并预测代谢途径。我们的结果表明,ESCC,EGJ和ESCC后组的微生物多样性明显低于HC组。Fusobacteria的丰度更高(7.01比1.12%,P= 0.039),ESCC组的放线菌丰度(1.61比4.04%)低于HC组。我们发现拟杆菌的丰度存在显着差异(20.45比9.86%,P = 0.026),梭菌(7.01对1.66%, P= 0.030)。微生物成分分析和PICRUSt结果表明ESCC组和HC组之间存在显着差异。β多样性和PICRUSt提示食管切除术后的微生物组成和代谢途径与HC组相似。食道微生物群的监测可能是预测肿瘤复发的重要方法。

更新日期:2020-06-26
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