Adding biochar to soil can change soil properties and subsequently affect plant growth, but this effect can vary because of different feedstocks and methods (e.g., pyrolysis or gasification) used to create the biochar. Growth and biological nitrogen fixation (BNF) of leguminous plants can be improved with rhizobia inoculation that fosters nodule development. Thus, this factorial greenhouse study examined the effects of two types of biochar (i.e., pyrolysis and gasification) added at a rate of 5% (v:v) to a peat-based growth substrate and rhizobia inoculation (yes or no) on Robinia pseudoacacia (black locust) seedlings supplied with ¹⁵NH₄¹⁵NO₃. Seedling and nodule growth, nitrogen (N) content, and δ¹⁵N × 1000 were evaluated after 3 months. While addition of biochar without inoculation had no effect on seedling growth, inoculation with rhizobia increased seedling growth, BNF, and N status. Inoculated seedlings had reduced δ¹⁵N, indicating that N provided via fertilization was being diluted by N additions through BNF. Biochar type and inoculation interacted to affect seedling growth. Combining inoculation with either biochar type increased seedling leaf, stem, and total biomass, whereas gasifier biochar and inoculation improved all seedling growth variables and nodule biomass.

中文翻译：

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生物炭类型和根瘤菌接种的相互作用增加了刺槐幼苗的生长和生物固氮作用

向土壤中添加生物炭可以改变土壤特性并随后影响植物的生长，但是由于用于创建生物炭的原料和方法（例如热解或气化）不同，这种影响可能会有所不同。根瘤菌接种可促进根瘤发育，从而可以改善豆科植物的生长和生物固氮（BNF）。因此，本阶乘温室研究检查在5％的速率（加入的两种类型的生物炭的（即，热解和气化）的影响V：V）到基于泥炭的生长衬底和根瘤菌接种（是或否）刺槐供应¹⁵ NH ₄ ¹⁵ NO _3的拟槐（黑刺槐）幼苗。幼苗和结核生长，氮（N）含量，和δ ¹⁵ N×1000 3个月后进行评价。虽然添加未经接种的生物炭对幼苗生长没有影响，但根瘤菌接种增加了幼苗的生长，BNF和N状态。接种的幼苗具有降低的δ ¹⁵ N，指示N个提供经由受精是通过氮添加通过BNF稀释。生物炭类型和接种相互作用影响幼苗的生长。接种两种生物炭类型均可增加幼苗叶片，茎和总生物量，而气化炉生物炭和接种方法可改善所有幼苗生长变量和根瘤生物量。