The aim of this study was to fabricate antimicrobial calcium‐alginate‐based films containing the self‐microemulsifying thyme essential oil (TEO) formulations using Tween 80 as the surfactant, and acetic (AA) or propionic (PA) acids as the cosurfactants. A Ca‐alginate film containing nano‐emulsified TEO as well as a neat Ca‐alginate film were considered as the controls. The scanning electron microscopy micrographs showed a highly porous texture for SME films, which resulted in an increase in water vapor permeability and water absorption capacity of these films. The SME films released the TEO completely within 155 min and inhibited the growth of S. aureus and E. coli in in vitro antimicrobial tests. The population of S. aureus and E. coli reduced significantly in ground beef covered with SME films. The results of this study showed that self‐microemulsifying TEO films could effectively increase the shelf life of ground beef by controlling its microbial population.

中文翻译：

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含自微乳化百里香精油的海藻酸钙薄膜的释放速率和抗菌活性对大肠杆菌和金黄色葡萄球菌的影响

这项研究的目的是使用Tween 80作为表面活性剂，乙酸（AA）或丙酸（PA）酸作为助表面活性剂，制备包含自微乳化百里香精油（TEO）配方的抗菌藻酸钙基薄膜。包含纳米乳化TEO的钙藻酸盐薄膜以及纯净的钙藻酸盐薄膜被视为对照。扫描电子显微镜显微照片显示SME膜具有高度多孔的质地，这导致这些膜的水蒸气渗透性和吸水能力增加。在体外抗菌测试中， SME薄膜在155分钟内完全释放了TEO，并抑制了金黄色葡萄球菌和大肠杆菌的生长。人口金黄色葡萄球菌和覆盖有SME薄膜的碎牛肉中的大肠杆菌显着减少。这项研究的结果表明，自微乳化的TEO膜可以通过控制其微生物种群有效地延长碎牛肉的保质期。