Abstract The success of biomaterials depends on several factors that determine their proper interaction with the biological environment. Their physical and chemical properties, such as the rigidity of the substrate and the topography and chemistry of the surface, have an important influence on cellular behavior. This work describes the modification of the surface of polyethylenterephthalate (PET) films with primary amino groups, as well as cell adhesion tests on these surfaces. Herein, gamma radiation was used to graft acryloyl chloride (AC) by an oxidative preirradiation method to obtain PET-g-AC with a grafting percent of 4.8 ± 0.07%. After grafting ethylenediamine (ED) was reacted with PET-g-AC via an aminolysis reaction to generate PET-g-EAAm where NH2 terminal groups are present on the polymer surface. The films were characterized by contact angle, FT-IR, TGA, SEM and AFM. The presence of NH2 groups on modified PET films’ surfaces was confirmed by XPS, and the amount (20.3 + 0.7 pmol mm-2) was calculated using a colorimetric method; this quantity was determined to be sufficient to carry out cell culture assays, demonstrating that PET-g-EAAm films with primary amines are biocompatible with epithelial and mesenchymal cells lines, which play a very important role in the development and design of new materials for biomedical applications.

中文翻译：

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使用伽马辐射和氨解反应用伯胺对聚对苯二甲酸乙二酯薄膜进行表面改性，用于细胞粘附研究

摘要 生物材料的成功取决于决定其与生物环境适当相互作用的几个因素。它们的物理和化学特性，例如基材的刚度和表面的形貌和化学性质，对细胞行为有重要影响。这项工作描述了用伯氨基对聚对苯二甲酸乙二醇酯 (PET) 薄膜的表面进行改性，以及在这些表面上进行细胞粘附测试。本文采用γ射线通过氧化预辐照法接枝丙烯酰氯(AC)，得到接枝率为4.8±0.07%的PET-g-AC。接枝乙二胺 (ED) 后，通过氨解反应与 PET-g-AC 反应生成 PET-g-EAAm，其中 NH2 端基存在于聚合物表面。通过接触角、FT-IR、TGA、SEM和AFM对薄膜进行表征。通过XPS确认改性PET薄膜表面存在NH2基团，并使用比色法计算其量（20.3 + 0.7 pmol mm-2）；该数量被确定足以进行细胞培养试验，证明含有伯胺的 PET-g-EAAm 薄膜与上皮和间充质细胞系具有生物相容性，这在生物医学新材料的开发和设计中起着非常重要的作用。应用程序。