BACKGROUND Diabetes mellitus is the disease, termed either by insulin paucity or resistance and hyperglycemia. The selection of the cerebellum was built on its specific functions. The aim of this study was to investigate a comparison between the possible therapeutic effects of MSCs and curcumin against fluctuations in the cerebellar cortex of STZ-induced diabetic albino rats. MATERIALS AND METHODS Forty rats were divided into five groups: control, sham control, streptozotocin-induced diabetes, diabetes and MSCs administered and diabetes and curcumin administered. Light microscopic (H&E), immune-histochemical; Glial fibrillary acidic protein (GFAP), real-time PCR (phospholipase-C (PLC) and α-synuclein), histomorphometric analysis, oxidative / anti-oxidatants; malondialdehyde (MDA)/ superoxide dismutase (SOD) glutathione (GSH) and were made. RESULTS The histopathological examination of the STZ-induced diabetic rats revealed alterations in the molecular, purkinje and granular layers. Abnormal organizations, vacuolation, patchy loss of purkinje cells were detected. Some purkinje cells migrated into the granular layer.Hemorrhage in pia mater outspreading to cerebellar layers is discerned. Purkinje cells showed karyorrhexis. The mean value of area percentage for GFAP and procasapase immune- reactivity revealed 360 % and 400 % significant increase compared to those of the control group. Also, MDA level was significantly increased while the SOD and GSH levels were significantly lower when compared to the control group. Meanwhile, mean values of PLC demonstrated significant rise, while α-synuclein levels displayed a significant decrement in the diabetic group. Administration of curcumin and MSCs extremely ameliorated the previous alterations. CONCLUSION the deleterious alterations on the cerebellar cortex induced by diabetes were obviously improved when treated with either MSCs or curcumin.

中文翻译：

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间充质干细胞与姜黄素在增强链脲佐菌素诱导的糖尿病白化大鼠小脑皮层的改变中的作用。GFAP、PLC和α-突触核蛋白的作用

背景糖尿病是一种疾病，被称为胰岛素缺乏或抵抗和高血糖症。小脑的选择建立在其特定的功能之上。本研究的目的是比较 MSCs 和姜黄素对 STZ 诱导的糖尿病白化大鼠小脑皮层波动的可能治疗效果。材料与方法 将 40 只大鼠分为 5 组：对照组、假手术组、链脲佐菌素诱导的糖尿病组、糖尿病和 MSCs 组以及糖尿病和姜黄素组。光学显微镜 (H&E)，免疫组化；胶质纤维酸性蛋白 (GFAP)、实时 PCR（磷脂酶-C (PLC) 和 α-突触核蛋白）、组织形态计量分析、氧化/抗氧化剂；丙二醛 (MDA)/超氧化物歧化酶 (SOD) 谷胱甘肽 (GSH) 并制成。结果 STZ诱导的糖尿病大鼠的组织病理学检查显示分子层、浦肯野层和颗粒层的改变。检测到异常组织、空泡化、浦肯野细胞斑片状丢失。一些浦肯野细胞迁移到颗粒层。可见软脑膜出血扩展到小脑层。浦肯野细胞显示核破裂。GFAP 和 procasapase 免疫反应性的面积百分比平均值显示，与对照组相比，显着增加了 360% 和 400%。此外，与对照组相比，MDA 水平显着增加，而 SOD 和 GSH 水平显着降低。同时，PLC 的平均值显示出显着上升，而 α-突触核蛋白水平在糖尿病组中显示出显着下降。姜黄素和 MSCs 的给药极大地改善了以前的改变。结论 MSCs或姜黄素对糖尿病引起的小脑皮质有害改变均有明显改善。