Purpose

The rapid detection of carbapenemases among Enterobacterales in clinical laboratories is critical for management of patients, and infection prevention and control efforts.

Methods

A study was designed to evaluate the performances of the RAPIDEC CARBA NP®, β-CARBA®, NG-Test CARBA 5®, modified carbapenem-inactivation method, and EDTA version (eCIM) assays against a global collection of Enterobacterales (n = 216) with diverse carbapenemases.

Results

The RAPIDEC CARBA NP® assay had a sensitivity of 98.6% and specificity of 19.6% and β-CARBA® a sensitivity of 94% and specificity of 97.8%, but showed low sensitivity with Klebsiella Pneumoniae Carbapenemase (KPC)-containing isolates. The NG-Test CARBA 5® had an overall sensitivity of 96.3% and specificity of 100% and failed to detect isolates with bla_IMP-13, bla_IMP-14. The eCIM gave false- positive results with Oxacillinase (OXA)-48–like enzymes.

Conclusion

The NG-Test CARBA 5® assay was technically simple and provided rapid accurate results on the types of carbapenemases. Such information has potential treatment benefits for patients.

中文翻译：

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快速检测产生碳青霉烯酶的肠杆菌。

目的

在临床实验室中，快速检测肠杆菌中的碳青霉烯酶对患者的管理以及感染的预防和控制工作至关重要。

方法

设计一项研究来评估RAPIDEC CARBANP®，β-CARBA®，NG-Test CARBA5®，改良的碳青霉烯灭活方法和EDTA版（eCIM）检测对肠杆菌的全球收集量的性能（n  = 216） ）与各种碳青霉烯酶。

结果

RAPIDEC CARBANP®测定的灵敏度为98.6％，特异性为19.6％，β-CARBA®的灵敏度为94％，特异性为97.8％，但对于含肺炎克雷伯菌碳青霉烯酶（KPC）的分离株，灵敏度较低。NG-Test CARBA5®的总灵敏度为96.3％，特异性为100％，无法检测到bla _IMP-13，bla _IMP-14分离株。eCIM使用类似Oxacillinase（OXA）-48的酶产生假阳性结果。

结论

NG-Test CARBA5®检测技术简单，可对碳青霉烯酶的类型提供快速准确的结果。这些信息对患者具有潜在的治疗益处。