当前位置: X-MOL 学术Curr. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila.
Current Biology ( IF 9.2 ) Pub Date : 2020-06-25 , DOI: 10.1016/j.cub.2020.05.078
Jeroen Dobbelaere 1 , Marketa Schmidt Cernohorska 2 , Martina Huranova 2 , Dea Slade 1 , Alexander Dammermann 1
Affiliation  

Centrioles are highly elaborate microtubule-based structures responsible for the formation of centrosomes and cilia. Despite considerable variation across species and tissues within any given tissue, their size is essentially constant [1, 2]. While the diameter of the centriole cylinder is set by the dimensions of the inner scaffolding structure of the cartwheel [3], how centriole length is set so precisely and stably maintained over many cell divisions is not well understood. Cep97 and CP110 are conserved proteins that localize to the distal end of centrioles and have been reported to limit centriole elongation in vertebrates [4, 5]. Here, we examine Cep97 function in Drosophila melanogaster. We show that Cep97 is essential for formation of full-length centrioles in multiple tissues of the fly. We further identify the microtubule deacetylase Sirt2 as a Cep97 interactor. Deletion of Sirt2 likewise affects centriole size. Interestingly, so does deletion of the acetylase Atat1, indicating that loss of stabilizing acetyl marks impairs centriole integrity. Cep97 and CP110 were originally identified as inhibitors of cilia formation in vertebrate cultured cells [6], and loss of CP110 is a widely used marker of basal body maturation. In contrast, in Drosophila, Cep97 appears to be only transiently removed from basal bodies and loss of Cep97 strongly impairs ciliogenesis. Collectively, our results support a model whereby Cep97 functions as part of a protective cap that acts together with the microtubule acetylation machinery to maintain centriole stability, essential for proper function in cilium biogenesis.



中文翻译:

Cep97 是果蝇中心粒结构完整性和纤毛形成所必需的。

中心粒是高度精细的基于微管的结构,负责形成中心体和纤毛。尽管在任何给定组织内的物种和组织之间存在相当大的差异,但它们的大小基本上是恒定的 [1, 2]。虽然中心粒圆柱的直径是由车轮的内部脚手架结构的尺寸设置的 [3],但中心粒长度如何在许多细胞分裂中如此精确和稳定地设置还不清楚。Cep97 和 CP110 是位于中心粒远端的保守蛋白,据报道可限制脊椎动物的中心粒伸长 [4, 5]。在这里,我们检查了黑腹果蝇中的Cep97 功能. 我们表明,Cep97 对于在苍蝇的多个组织中形成全长中心粒是必不可少的。我们进一步将微管脱乙酰酶 Sirt2 鉴定为 Cep97 相互作用物。删除 Sirt2 同样会影响中心粒大小。有趣的是,乙酰酶 Atat1 的缺失也是如此,这表明稳定乙酰标记的缺失损害了中心粒的完整性。Cep97 和 CP110 最初被确定为脊椎动物培养细胞中纤毛形成的抑制剂 [6],而 CP110 的缺失是一种广泛使用的基体成熟标志物。相比之下,在果蝇中,Cep97 似乎只是暂时从基体中去除,Cep97 的缺失会严重损害纤毛发生。总的来说,我们的结果支持一个模型,其中 Cep97 作为保护帽的一部分,与微管乙酰化机制一起作用以维持中心粒稳定性,这对于纤毛生物发生的正常功能至关重要。

更新日期:2020-08-03
down
wechat
bug