Binding effect and interaction of 2-pentadecanoyloxymethyl)trimethylammonium bromide (DMGM-14) with bovine serum albumin (BSA) and hen egg white lysozyme (HEWL) were systematically investigated by the fluorescence spectroscopy, circular dichroism (CD) spectroscopy, isothermal titration calorimetry (ITC), surface tension analysis, and molecular docking studies. The emulsion properties and particle size distribution of surfactant/protein complexes containing sunflower oil were studied using static light scattering and confocal laser scanning microscopy (CLSM). The fluorescence spectroscopy and ITC analysis confirmed the complexes formation of DMGM-14 with BSA and HEWL which was also verified by surface tension measurements. CD results explained the conformational changes in BSA and HEWL upon DMGM-14 complexation. Molecular docking study provides insight into the binding of DMGM-14 into the specific sites of BSA and HEWL. Besides, the studies drew a detailed picture on the emulsification properties of DMGM-14 with BSA and HEWL. In addition, the in vitro experiment revealed a broad antibacterial spectrum of DMGM-14 and DMGM-14/HEWL complex including activity against Gram-positive and Gram-negative bacteria. In conclusion, the present study revealed that the interaction between DMGM-14 with BSA and HEWL is important for the pharmaceutical, biological, and food products.

通过荧光光谱，圆二色性（CD）光谱，等温滴定量热法（系统分析）系统研究了2-戊二酰氧基甲基）三甲基溴化铵（DMGM-14）与牛血清白蛋白（BSA）和鸡蛋清溶菌酶（HEWL）的结合作用和相互作用。 ITC），表面张力分析和分子对接研究。使用静态光散射和共聚焦激光扫描显微镜（CLSM）研究了含葵花籽油的表面活性剂/蛋白质复合物的乳液性能和粒度分布。荧光光谱和ITC分析证实了DMGM-14与BSA和HEWL的复合物形成，这也通过表面张力测量得到证实。CD结果解释了DMGM-14复合后BSA和HEWL的构象变化。分子对接研究可深入了解DMGM-14与BSA和HEWL特定位点的结合。此外，研究还对BSGM和HEWL对DMGM-14的乳化性能进行了详细描述。除此之外体外实验显示，DMGM-14和DMGM-14 / HEWL复合物具有广泛的抗菌谱，包括对革兰氏阳性和革兰氏阴性细菌的活性。总之，本研究表明DMGM-14与BSA和HEWL之间的相互作用对于药物，生物和食品非常重要。