Nowadays, single cell oil (SCO) can play two distinct roles, first as a supplier of functional oils, and second as a feedstock for the biodiesel industry. These two distinct functions require a different fatty acids (FA) profile in the lipid pool. Moreover, to exploit their potential for industrialization, it is necessary to employ a low-cost substrate. Crude glycerol is the main side-product of biodiesel production. This renewable feedstock is one of Yarrowia lipolytica favorable substrates. In this study we improved polyunsaturated fatty acids (PUFA) synthesis by overexpression of the glycerol phosphate acyltransferase gene (SCT1). Here, we established a method to alter the quantity and FA composition of SCO. The engineered strain showed a 10-fold improvement (>20%) in linoleic acid synthesis (C18:2) in a shake-flask experiment. In a fermenter study co-overexpression of glycerol kinase (GUT1) and SCT1 allowed for 3-fold improvement in C18:2 synthesis from crude glycerol and at low pH.

如今，单细胞油（SCO）可以扮演两个不同的角色，首先是功能油的供应商，其次是生物柴油行业的原料。这两个不同的功能在脂质池中需要不同的脂肪酸（FA）谱。此外，为了开发它们的工业化潜力，必须采用低成本的基板。粗甘油是生物柴油生产的主要副产品。该可再生原料是解脂耶氏酵母的有利底物之一。在这项研究中，我们通过甘油磷酸酰基转移酶基因（SCT1的过表达）改善了多不饱和脂肪酸（PUFA）的合成）。在这里，我们建立了改变上合组织数量和FA组成的方法。在摇瓶实验中，工程菌株显示出亚油酸合成（C18：2）改善了10倍（> 20％）。在发酵罐研究中，甘油激酶（GUT1）和SCT1的共过量表达使粗甘油和低pH条件下的C18：2合成提高了3倍。