Thousands of genes are involved in spermatogenesis, however, the functional roles of most these genes for male fertility remain to be discovered. This research focused to explore the function of evolutionarily conserved and testis-specific expressed gene 4930524B15Rik, which is known as C5orf47 in human. We generated 4930524B15Rik knockout mice by CRISPR/Cas9 technology and found 4930524B15Rik^−/− mice were fertile. Furthermore, no averted abnormalities were observed in testis morphology, epididymal sperm contents and sperm morphology in 4930524B15Rik knockout mice. Subsequently, histological analysis of testicular tissue revealed intact structure of seminiferous tubules along with the presence of all types of germ cells in 4930524B15Rik^−/− mice similar to wild type. Additionally, cytological analysis of spermatocytes displayed no significant differences in the prophase I progression of meiosis, further indicating that 4930524B15Rik have no essential function in mammalian spermatogenesis. Altogether, these results indicated that 4930524B15Rik is dispensable for fertility of male mice and these findings will help researchers to avoid future research overlap and to focus on genes that are crucial for spermatogenesis and reproduction.

精子发生涉及成千上万的基因，然而，大多数这些基因对男性生育的功能作用仍有待发现。这项研究的重点是探索进化保守和睾丸特异性表达的基因4930524B15Rik的功能，该基因在人类中被称为C5orf47。我们通过CRISPR / Cas9技术生成了4930524B15Rik敲除小鼠，发现4930524B15Rik ^-/-小鼠可育。此外，在4930524B15Rik中未观察到睾丸形态，附睾精子含量和精子形态的避免异常。剔除小鼠。随后，在4930524B15Rik ^-/-小鼠中，与野生型相似，睾丸组织的组织学分析显示出生精小管的完整结构以及所有类型生殖细胞的存在。此外，精母细胞的细胞学分析显示减数分裂的前期I进程没有显着差异，进一步表明4930524B15Rik在哺乳动物的精子发生中没有必要的功能。总而言之，这些结果表明4930524B15Rik对于雄性小鼠的生育是必不可少的，这些发现将有助于研究人员避免将来的研究重叠，并专注于对精子发生和繁殖至关重要的基因。