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Life in mine tailings: microbial population structure across the bulk soil, rhizosphere, and roots of boreal species colonizing mine tailings in northwestern Québec
Annals of Microbiology ( IF 3 ) Pub Date : 2020-06-24 , DOI: 10.1186/s13213-020-01582-9
Vanessa Gagnon , Michaël Rodrigue-Morin , Julien Tremblay , Jessica Wasserscheid , Julie Champagne , Jean-Philippe Bellenger , Charles W. Greer , Sébastien Roy

Mining activities have negative effects on soil characteristics and can result in low pH, high heavy metal content, and limited levels of essential nutrients. A tailings storage area located in northwestern Québec showed natural colonization by plants from the adjacent natural environment. The objective of the study was to determine the main edaphic parameters that structured microbial populations associated with the indigenous woody plants that had naturally colonized the site. Microbial populations were studied in the bulk soil, the rhizosphere, and inside plant roots using Illumina sequencing, ordination analysis (i.e., redundancy analysis (RDA) and principal coordinates analysis (PCoA)), ternary plotting, and statistical analysis (MANOVA). The main variables that drove the microbial community patterns were plant species and the tailings pH. Indeed, the main bacterial classes were Gammaproteobacteria and Deltaproteobacteria in both the rhizosphere and root endosphere. Analysis revealed that some dominant operational taxonomic units (e.g., Pseudomonas sp., Acinetobacter sp., and Delftia sp.) were present in increased proportions in roots for each plant species under study. This study also revealed that many of the most abundant fungal genera (e.g., Claussenomyces, Eupenicillium, and Trichoderma) were more abundant in the rhizosphere than in the root endosphere. This comprehensive study of the microbial community dynamics in the bulk soil, rhizosphere, and root endosphere of boreal trees and shrubs could be beneficial in facilitating the rehabilitation of disturbed ecosystems.

中文翻译:

矿山尾矿中的生活:魁北克西北部遍及土壤,根际和在矿山尾部定居的北方物种根系的微生物种群结构

采矿活动对土壤特性有负面影响,并可能导致pH值低,重金属含量高和必需营养素含量有限。魁北克西北部的一个尾矿存储区显示出邻近自然环境中的植物对它们的自然定殖。这项研究的目的是确定构成与自然定殖的本地木本植物有关的微生物种群的主要营养参数。使用Illumina测序,排序分析(即冗余分析(RDA)和主坐标分析(PCoA)),三元绘图和统计分析(MANOVA),研究了大块土壤,根际和植物根内部的微生物种群。驱动微生物群落模式的主要变量是植物种类和尾矿pH。的确,根际和根内球面的主要细菌种类为γ-变形杆菌和δ-变形杆菌。分析表明,每种研究植物的根中都存在一些主要的操作分类单位(例如,假单胞菌属,不动杆菌属和德尔福特属)。这项研究还表明,根际内的根际层比根际内的根层更丰富。许多最丰富的真菌属(例如克劳氏菌,大戟属和木霉属)。对北方树木和灌木的土壤,根际和根际内微生物群落动态的综合研究可能对促进受干扰生态系统的恢复有益。根际和根内圈的主要细菌种类为γ-变形杆菌和δ-变形杆菌。分析表明,每种研究植物的根中都存在一些主要的操作分类单位(例如,假单胞菌属,不动杆菌属和德尔福特属)。这项研究还表明,根际内的根际层比根际内的根层更丰富。许多最丰富的真菌属(例如克劳氏菌,大戟属和木霉属)。对北方树木和灌木的土壤,根际和根际内微生物群落动态的综合研究可能对促进受干扰生态系统的恢复有益。根际和根内圈的主要细菌种类为γ-变形杆菌和δ-变形杆菌。分析表明,每种研究植物的根中都存在一些主要的操作分类单位(例如,假单胞菌属,不动杆菌属和德尔福特属)。这项研究还表明,根际内的根际层比根际内的根层更丰富。许多最丰富的真菌属(例如克劳氏菌,大戟属和木霉属)。这项对寒带树木和灌木丛的土壤,根际和根部内层微生物群落动态的综合研究可能对促进受干扰生态系统的恢复有益。,和Delftia sp。)在研究中的每种植物的根中都以增加的比例存在。这项研究还表明,根际中许多丰富的真菌属(例如克劳氏菌,大戟属和木霉属)比根部内层中的丰富得多。这项对寒带树木和灌木丛的土壤,根际和根部内层微生物群落动态的综合研究可能对促进受干扰生态系统的恢复有益。,和Delftia sp。)在研究中的每种植物的根中都以增加的比例存在。这项研究还表明,根际内的根际层比根际内的根层更丰富。许多最丰富的真菌属(例如克劳氏菌,大戟属和木霉属)。对北方树木和灌木的土壤,根际和根际内微生物群落动态的综合研究可能对促进受干扰生态系统的恢复有益。
更新日期:2020-06-25
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