Understanding the relationships among multiple ecosystem services could improve the landscape capacity to provide benefits to human society. However, the underlying mechanisms shaping ecosystem services relationships are still unclear although some studies have been conducted to explore how natural and socioeconomic factors influence the relationships among ecosystem services. In this study, the karst landscape in southwestern China, a vulnerable system with intensive human activities, was focused on, aiming to explore relationships between ecosystem services and associated social and ecological factors. The results showed that the distribution of eight individual ecosystem services were spatially heterogeneous and clustered based on the characteristics of the karst landscape. The relationships between provisioning services and regulating services, such as grain production and net primary productivity, as well as water yield and soil retention, were quite different in high karst coverage regions and low karst coverage regions. Among five ecosystem service bundles identified, ecosystem services in the urban development bundle were mainly determined by socioeconomic factors, while in the other four bundles of multifunction, grain production, habitat conservation, and carbon sequestration, ecosystem services were dominated by ecological factors. However, socioeconomic factors (i.e. population density and night-time light intensity) appeared to explain the overall ecosystem service delivery more than karst terrain. This study provided insights for sustainable ecosystem management in a vulnerable karst region through exploring social-ecological factors of the relationships among ecosystem services.