Fiber-optic-based two-photon fluorescence endomicroscopy is emerging as an enabling technology for in vivo histological imaging of internal organs and functional neuronal imaging on freely-behaving animals. However, high-speed imaging remains challenging due to the expense of miniaturization and lack of suited fast beam scanners. For many applications, a higher imaging speed is highly desired, especially for monitoring functional dynamics such as transient dendritic responses in neuroscience. This Letter reports the development of a fast fiber-optic scanning endo-microscope with an imaging speed higher than 26 frames/s. In vivo neural dynamics imaging with the high-speed endomicroscope was performed on a freely-behaving mouse over the primary motor cortex that expressed GCaMP6m. The results demonstrate its capability of real-time monitoring of transient neuronal dynamics with very fine temporal resolution.

中文翻译：

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用于体内神经元动力学成像的高速光纤扫描非线性内窥镜检查。

基于光纤的双光子荧光内窥镜技术正在成为一种使人能够对内部器官进行体内组织学成像以及对行为自由的动物进行功能性神经元成像的使能技术。然而，由于小型化的代价和缺乏合适的快速光束扫描仪，高速成像仍然具有挑战性。对于许多应用，非常需要更高的成像速度，尤其是对于监视功能动力学（例如神经科学中的瞬时树突响应）。这封信报道了成像速度高于26帧/秒的快速光纤扫描内窥镜的开发。体内高速内窥镜对神经动力学成像是在表现GCaMP6m的主运动皮层上的行为自由的小鼠上进行的。结果证明了其具有非常精细的时间分辨率的实时监测瞬时神经元动力学的能力。