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Optogenetic activation of Type III taste cells modulates taste responses.
Chemical Senses ( IF 3.5 ) Pub Date : 2020-06-25 , DOI: 10.1093/chemse/bjaa044
Aurelie Vandenbeuch 1 , Courtney E Wilson 1 , Sue C Kinnamon 1
Affiliation  

Studies have suggested that communication between taste cells shapes the gustatory signal before transmission to the brain. To further explore the possibility of intragemmal signal modulation, we adopted an optogenetic approach to stimulate sour-sensitive (Type III) taste cells using mice expressing Cre recombinase under a specific Type III cell promoter, Pkd2l1 (polycystic kidney disease-2-like 1), crossed with mice expressing Cre-dependent channelrhodopsin (ChR2). The application of blue light onto the tongue allowed for the specific stimulation of Type III cells and circumvented the nonspecific effects of chemical stimulation. To understand whether taste modality information is preprocessed in the taste bud before transmission to the sensory nerves, we recorded chorda tympani nerve activity during light and/or chemical tastant application to the tongue. To assess intragemmal modulation, we compared nerve responses to various tastants with or without concurrent light-induced activation of the Type III cells. Our results show that light significantly decreased taste responses to sweet, bitter, salty, and acidic stimuli. On the contrary, the light response was not consistently affected by sweet or bitter stimuli, suggesting that activation of Type II cells does not affect nerve responses to stimuli that activate Type III cells.

中文翻译:

III型味觉细胞的光遗传学激活可调节味觉反应。

研究表明,味觉细胞之间的通讯会在味觉信号传递到大脑之前形成味觉信号。为了进一步探索内胚层信号调节的可能性,我们采用了光遗传学方法,使用在特定的III型细胞启动子Pkd2l1下表达Cre重组酶的小鼠刺激酸敏感(III型)味觉细胞(多囊肾疾病2-1)与表达Cre依赖性通道视紫红质(ChR2)的小鼠杂交。在舌头上施加蓝光可以特异性刺激III型细胞,并避免了化学刺激的非特异性作用。为了了解味觉信息是否在传递给感觉神经之前在味蕾中进行了预处理,我们记录了在味觉和/或化学味觉应用到舌头期间的鼓膜鼓膜神经活动。为了评估胚内调节,我们比较了有或没有同时发生光诱导的III型细胞活化的各种口味的神经反应。我们的结果表明,光显着降低了对甜,苦,咸和酸性刺激的味觉响应。反之,
更新日期:2020-06-25
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