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Injectable Electrical Conductive and Phosphate Releasing Gel with Two-Dimensional Black Phosphorus and Carbon Nanotubes for Bone Tissue Engineering
ACS Biomaterials Science & Engineering ( IF 5.8 ) Pub Date : 2020-06-24 , DOI: 10.1021/acsbiomaterials.0c00612
Xifeng Liu 1, 2 , Matthew N George 1, 2 , Linli Li 1, 2 , Darian Gamble 1, 2 , A Lee Miller Ii 2 , Bipin Gaihre 1, 2 , Brian E Waletzki 2 , Lichun Lu 1, 2
Affiliation  

Injectable hydrogels have unique advantages for the repair of irregular tissue defects. In this study, we report a novel injectable carbon nanotube (CNT) and black phosphorus (BP) gel with enhanced mechanical strength, electrical conductivity, and continuous phosphate ion release for tissue engineering. The gel utilized biodegradable oligo(poly(ethylene glycol) fumarate) (OPF) polymer as the cross-linking matrix, with the addition of cross-linkable CNT-poly(ethylene glycol)-acrylate (CNTpega) to grant mechanical support and electric conductivity. Two-dimensional (2D) black phosphorus nanosheets were also infused to aid in tissue regeneration through the steady release of phosphate that results from environmental oxidation of phosphorus in situ. This newly developed BP-CNTpega-gel was found to enhance the adhesion, proliferation, and osteogenic differentiation of MC3T3 preosteoblast cells. With electric stimulation, the osteogenesis of preosteoblast cells was further enhanced with elevated expression of several key osteogenic pathway genes. As monitored with X-ray imaging, the BP-CNTpega-gel demonstrated excellent in situ gelation and cross-linking to fill femur defects, vertebral body cavities, and posterolateral spinal fusion sites in the rabbit. Together, these results indicate that this newly developed injectable BP-CNTpega-gel owns promising potential for future bone and broad types of tissue engineering applications.

中文翻译:

用于骨组织工程的具有二维黑磷和碳纳米管的可注射导电和磷酸盐释放凝胶

可注射水凝胶在修复不规则组织缺损方面具有独特的优势。在这项研究中,我们报告了一种新型可注射碳纳米管 (CNT) 和黑磷 (BP) 凝胶,具有增强的机械强度、导电性和用于组织工程的连续磷酸离子释放。该凝胶利用可生物降解的低聚(聚(乙二醇)富马酸酯)(OPF)聚合物作为交联基质,并添加了可交联的 CNT-聚(乙二醇)-丙烯酸酯(CNTpega)以提供机械支撑和导电性. 还注入了二维 (2D) 黑磷纳米片,以通过磷的原位环境氧化产生的磷酸盐的稳定释放来帮助组织再生。发现这种新开发的 BP-CNTpega-gel 可以增强粘附、增殖、和 MC3T3 前成骨细胞的成骨分化。通过电刺激,随着几个关键成骨途径基因的表达升高,前成骨细胞的成骨能力进一步增强。通过 X 射线成像监测,BP-CNTpega 凝胶表现出出色的原位凝胶化和交联作用,可填充兔子的股骨缺损、椎体腔和后外侧脊柱融合部位。总之,这些结果表明,这种新开发的可注射 BP-CNTpega 凝胶在未来的骨骼和广泛类型的组织工程应用中具有广阔的潜力。BP-CNTpega-gel 表现出优异的原位凝胶化和交联作用,可填充兔子的股骨缺损、椎体腔和后外侧脊柱融合部位。总之,这些结果表明,这种新开发的可注射 BP-CNTpega 凝胶在未来的骨骼和广泛类型的组织工程应用中具有广阔的潜力。BP-CNTpega-gel 表现出优异的原位凝胶化和交联作用,可填充兔子的股骨缺损、椎体腔和后外侧脊柱融合部位。总之,这些结果表明,这种新开发的可注射 BP-CNTpega 凝胶在未来的骨骼和广泛类型的组织工程应用中具有广阔的潜力。
更新日期:2020-08-10
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