Pain, physical dysfunction, and mental disorders caused by bone nonunion bring great burden to patients. Bone mesenchymal stem cells (BMSCs) isolated from bone nonunion patients with poor proliferation and osteogenic ability are compared with that from normal bone-healing patients. Long noncoding RNAs (lncRNAs) are a class of RNAs that are more than 200 nucleotides in length, lack an open-reading frame encoding a protein, and have little or no protein-coding function, and could regulate gene expression, which is involved in the regulation of important life activities, such as growth, development, aging, and death at epigenetic, transcriptional, and post-transcriptional levels. In this study, we intended to investigate the difference of lncRNA expression between patients with nonunion and normal fracture healing. Our result found that lncRNA ENST00000563492 was downregulated in bone nonunion tissues. LncRNA ENST00000563492 promotes osteogenic differentiation of BMSCs through upregulating the expression of CDH11. On the other hand, LncRNA ENST0000563492 could improve the osteogenesis–angiogenesis coupling process through enhancing the expression of VEGF during osteogenic differentiation of BMSCs. LncRNA ENST00000563492 functions as a ceRNA for miR-205-5p that was targeting CDH11 and VEGF. LncRNA ENST00000563492 could promote the osteogenesis of BMSCs in vivo. Our result indicated that lncRNA ENST00000563492 may be a new target for bone nonunion.

骨不愈合引起的疼痛，身体机能障碍和精神障碍给患者带来很大负担。将从增殖和成骨能力差的骨不愈合患者中分离出的骨间充质干细胞（BMSCs）与正常骨愈合患者进行比较。长非编码RNA（lncRNA）是一类RNA，长度超过200个核苷酸，缺少编码蛋白质的开放阅读框，几乎没有或没有蛋白质编码功能，并且可以调节基因表达，这与重要生命活动的调控，例如表观遗传，转录和转录后水平的生长，发育，衰老和死亡。在这项研究中，我们打算调查不愈合患者和正常骨折愈合患者lncRNA表达的差异。我们的结果发现，lncRNA ENST00000563492在骨不愈合组织中被下调。LncRNA ENST00000563492通过上调CDH11的表达来促进BMSC的成骨分化。另一方面，LncRNA ENST0000563492可以通过增强BMSCs成骨分化过程中VEGF的表达来改善成骨-血管生成的偶联过程。LncRNA ENST00000563492用作针对CDH11和VEGF的miR-205-5p的ceRNA。LncRNA ENST00000563492可以在体内促进BMSCs的成骨作用。我们的结果表明，lncRNA ENST00000563492可能是骨不愈合的新靶标。LncRNA ENST0000563492可通过增强BMSCs成骨分化过程中VEGF的表达来改善成骨-血管生成偶联过程。LncRNA ENST00000563492用作针对CDH11和VEGF的miR-205-5p的ceRNA。LncRNA ENST00000563492可以在体内促进BMSCs的成骨作用。我们的结果表明，lncRNA ENST00000563492可能是骨不愈合的新靶标。LncRNA ENST0000563492可通过增强BMSCs成骨分化过程中VEGF的表达来改善成骨-血管生成偶联过程。LncRNA ENST00000563492用作针对CDH11和VEGF的miR-205-5p的ceRNA。LncRNA ENST00000563492可以在体内促进BMSCs的成骨作用。我们的结果表明，lncRNA ENST00000563492可能是骨不愈合的新靶标。