Abstract

Variability in the contents of representative secondary metabolites in Gentiana pneumonanthe individuals was investigated in relation to population origin, plant parts and developmental stage. Chemical analyses (HPLC) of six secondary metabolites (swertiamarin (SWM), gentiopicrin (GP), sweroside (SWZ), mangiferin (MGF), isoorientin (ISOOR), and isovitexin (ISOV)) were performed for individuals from six populations located within a 3 × 3 km area at Maljen Mt., and separately for below- and above ground plant parts in three developmental stages. Different plant parts showed pronounced differences in the contents of the six secondary metabolites and a high level of variability during the plant ontogeny. The highest contents of three dominant compounds (SWM, GP, and MGF) were detected in different developmental stages. Both below- and above ground plant parts contained GP, MGF, SWZ, and SWM at some developmental stages, whereas ISOOR and ISOV were registered in above ground parts. The full-flowering stage was characterized by the highest contents of MGF, GP, and ISOOR in aboveground-, and GP in belowground parts. In the post-flowering stage, a high content of MF was detected in below ground parts. The results of this study contribute to a better understanding of interpopulation, ontogenetic and between-organ variability in rare and threatened G. pneumonanthe.

摘要

龙胆香气菌中次生代谢产物含量的变异性对个体进行了有关种群起源，植物部位和发育阶段的调查。对来自六个人口中的六个人群的六个次生代谢产物（swertiamarin（SWM），gentiopicrin（GP），sweroside（SWZ），mangiferin（MGF），isoorientin（ISOOR）和isovitexin（ISOV）进行了化学分析（HPLC）位于Maljen山的3×3 km区域，在三个开发阶段分别用于地下和地面植物部分。在植物个体发育过程中，不同的植物部位显示出六种次生代谢产物的含量存在明显差异，并且具有高度的变异性。在不同的发育阶段检测到三种主要化合物（SWM，GP和MGF）的最高含量。在某些发育阶段，地下植物部分和地下植物部分均包含GP，MGF，SWZ和SWM，而ISOOR和ISOV已在地上部分注册。盛花期的特征是地上部分中的MGF，GP和ISOOR含量最高，地下部分中的GP含量最高。在开花后阶段，在地下部分检测到高含量的MF。这项研究的结果有助于更好地了解稀有和受威胁物种的种群间，个体发育和器官间变异性肺气单胞菌（G. pneumonanthe）。