ABSTRACT

Orexin-A (hypocretin-1) is a neuropeptide that is produced in the lateral hypothalamic area (LHA) and promotes widespread cortical activation. We investigated the effect of SB-334867, a selective orexin receptor 1 (OXR1) antagonist, on phospholipase C-β3 (PLCβ3) level using slices of rat hippocampus preparations and immunohistochemistry. We used three Wistar rats in each of three groups. The control group was untreated rats and SB vehicle and SB groups received SB vehicle and 10 mg/kg SB-334867 daily from postnatal day (PND) 12 to PND30, respectively. We found that the orexin receptor antagonist decreased the PLCβ3 level in the inner and outer blades of dentate gyrus (DG) compared to SB vehicle treated rats. Orexin may increase the PLCβ3 level in most regions of the rat hippocampus.

摘要

Orexin-A (hypocretin-1) 是一种在下丘脑外侧区 (LHA) 中产生的神经肽，可促进广泛的皮质激活。我们使用大鼠海马体切片和免疫组织化学研究了选择性食欲素受体 1 (OXR1) 拮抗剂 SB-334867 对磷脂酶 C-β3 (PLCβ3) 水平的影响。我们在三组中的每组中使用了三只 Wistar 大鼠。对照组是未治疗的大鼠，SB 载体和 SB 组分别从出生后第 12 天到 PND30 每天接受 SB 载体和 10 mg/kg SB-334867。我们发现，与 SB 载体处理的大鼠相比，食欲素受体拮抗剂降低了齿状回 (DG) 内外叶片中的 PLCβ3 水平。Orexin 可能会增加大鼠海马大部分区域的 PLCβ3 水平。