Abstract Conditions in dental unit waterlines are favourable for biofilm growth and contamination of dental unit water. The aim of this study was to assess the effect of several chemical disinfectants on bacteria in a biofilm model. Water-derived biofilms were grown in a static biofilm model (Amsterdam Active Attachment model), using two growth media. Biofilms were challenged with Alpron/Bilpron, Anoxyl, Citrisil, Dentosept, Green & Clean, ICX and Oxygenal in shock dose and maintenance doses. The concentration and the composition of the chemical disinfectants influenced the number of culturable bacteria in the biofilms. The application of a single shock dose followed by a low dose of the same chemical disinfectants resulted in the greatest suppression of viable bacteria in the biofilms. Exposure to Citrisil and ICX consistently resulted in failure to control the biofilms, while Alpron/Bilpron had a substantial and relevant effect on the number of bacteria in the biofilms.

中文翻译：

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牙科化学消毒剂对体外水源生物膜功效的异质性

摘要 牙科综合治疗台水线的条件有利于生物膜的生长和牙科综合治疗用水的污染。本研究的目的是评估几种化学消毒剂对生物膜模型中细菌的影响。水衍生的生物膜在静态生物膜模型（阿姆斯特丹主动附着模型）中生长，使用两种生长培养基。在休克剂量和维持剂量下，用 Alpron/Bilpron、Anoxyl、Citrisil、Dentosept、Green & Clean、ICX 和 Oxygenal 攻击生物膜。化学消毒剂的浓度和成分影响生物膜中可培养细菌的数量。使用单一冲击剂量，然后使用低剂量的相同化学消毒剂，可最大程度地抑制生物膜中的活细菌。