Abstract The use of hyperhalophilic microorganisms is emerging as a sustainable alternative to clean hydrocarbon-polluted hypersaline water bodies. In line with this practice, this work reports on the ability of the archaeon Halobacterium salinarum to develop biofilms on a solid surface conditioned by the presence of phenanthrene crystals, which results in the removal of the contaminating compound. The cell surface hydrophobicity does not change during the removal process and this organism is shown to constitutively produce a surfactant molecule with specific action on aromatic hydrocarbons, both indicating that phenanthrene removal might proceed through a non-contact mechanism. A new approach is presented to follow the process in situ through epifluorescence microscopy by monitoring phenanthrene auto-fluorescence. Graphical Abstract

中文翻译：

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盐酸盐细菌生物膜作为菲生物修复的工具

摘要 使用超嗜盐微生物正在成为清洁碳氢化合物污染的超咸水体的可持续替代品。与这种做法一致，这项工作报告了古生菌盐酸盐细菌在以菲晶体存在为条件的固体表面上形成生物膜的能力，从而去除污染化合物。细胞表面疏水性在去除过程中不会改变，并且该生物体显示出组成性地产生对芳烃具有特定作用的表面活性剂分子，这两者都表明菲去除可能通过非接触机制进行。提出了一种新方法，通过监测菲自发荧光，通过落射荧光显微镜原位跟踪该过程。图形概要