Eur. J. Immunol. 2008. 38: 2751–2761 DOI 10.1002/eji.200838542
Pulmonary stromal cells induce the generation of regulatory DC attenuating T‐cell‐mediated lung inflammation
Qian Li, Zhenhong Guo, Xiongfei Xu, Sheng Xia and Xuetao Cao
In the above‐mentioned article the D0, D6 and D12 fluorescent images of the “without stroma” group in Figure 1C were duplicates of the D0 “with stroma” data. In addition, in Figure 7C, the brightfield hematoxylin and eosin (H&E) images of lung sections of the “OVA+imDC” group was a duplication of the image of the “OVA+PBS” group. The authors have provided the original source data for Figure 1C and the corrected Figure 1 is provided below. For Figure 7C, the authors were unable to locate the original data for the experiment. As such, following a request by the editorial team, the authors have repeated the experiments presented in Figure 7C and have provided the raw data and an additional quantification for this figure. Quantification analysis was not part of the original publication; however, the Editors felt that such an analysis was essential in order to validate the choice of representative images. The corrected Figure 1 and Figure 7 are shown below along with the methods for the analysis. The raw data and analysis are included as Supporting Information. The repeated experiments in Figure 7 validate the conclusions of the paper.
Figure 1
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Co‐culture with MPSC promotes proliferation of imDC. (A) The morphology of MPSC under phase‐contrast light microscope (× 200; left panel) and the structure of MPSC under transmission electronic microscope (× 20 000; right panel). M, mitochondria; G, Golgi apparatus; ER, endoplasmic reticulum. (B) GFP+CD11c+ imDC at a density of 6 × 104 cells/well were added to the upper chamber of the 3‐mm transwell, with 0.7mL RPMI 1640, 10% MPSC SN, 50% MPSC SN and 100% MPSC SN, respectively, in the lower chamber. Two hours later, the cells in the lower chamber were recovered and counted by FACS. Data are presented as mean±SD of triplicate wells; ＊p<0.05. (C) The proliferation of GFP+CD11c+ imDC at a density of 3 × 104 cells/well in 24‐well plates in the presence of MPSC was monitored for 12 days (upper panel). GFP+CD11c+ imDC cultured in complete medium containing 10% FBS served as control (lower panel). Fluorescent photographs were taken every 6 days using a digital imaging system. Data are representative of three independent experiments. Scale bar 100 μm. (D) Single GFP+CD11c+ I‐A+ DC were sorted by FACS Diva onto the monolayer of MPSC and their proliferation was assessed at different time points. (E) GFP+CD11c+ imDC co‐cultured with MPSC for 0, 4 or 8 days were stained for I‐A, CD11b, CD11c, CD40, CD80 and CD86, respectively. All the experiments were repeated at least three times. Numbers in histograms indicate geometric mean fluorescence of test samples. Dotted lines represent control (unstained).
Figure 7
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Infusion of DCreg attenuates T‐cell‐mediated airway inflammation. Mice were immunized by i.p injection of chicken OVA adsorbed to alum adjuvant (day 0). Seven days later all mice were immunized again. PBS, imDC and DCreg were i.v. infused 6 days after the second immunization. At days 14–20 after the first immunization, mice were exposed daily to aerosolized PBS or OVA. (A‐B) BAL was performed 24 h after the last aerosol challenge. (A) Total cells were counted using a hemocytometer. Cytospin preparations of BAL cells were stained with Wright‐Giemsa to determine the differential cell counts. Data are presented as mean+ SD (n = 6 mice per experiment) and are from a single experiment representative of three experiments performed. Data analyses were performed using a two‐tailed Student's t‐test; *p<0.05 (B) Concentrations of IL‐4 and IL‐5 in the BAL fluid supernatant were assayed by ELISA. Data are presented as mean+ SD (n = 6 mice per experiment) and are from a single experiment representative of three experiments performed. Data analyses were performed using a two‐tailed Student's t‐test; *p<0.05. (C) 21 days after the first immunization, lungs were taken from the mice and inflammation quantified by counting the nuclei numbers close to the airway. The images (the upper panel) are the representative of each group and the lower panel is the quantification. The quantification data of nuclear counts were expressed as box plots and the median is indicated by the line in each box. Outliers are indicated by circles. Data are from a single experiment representative of three experiments performed with 5–6 mice per group/per experiment. The data analyses were performed using ANOVA and Tukey's multiple comparison tests; **p<0.01.
中文翻译：
更正。
欧元。J.免疫。2008. 38：2751–2761 DOI 10.1002 / eji.200838542
肺间质细胞诱导调节性DC减弱T细胞介导的肺部炎症的产生
李谦，郭振宏，徐雄飞，夏胜，曹雪涛
在上述文章中，图1C中“无基质”组的D0，D6和D12荧光图像是D0“有基质”数据的重复。另外，在图7C中，“ OVA + imDC”组的肺切片的明视野苏木和曙红（H＆E）图像是“ OVA + PBS”组的图像的重复。作者提供了图1C的原始源数据，并在下面提供了经过更正的图1。对于图7C，作者无法找到该实验的原始数据。这样，在编辑团队的要求下，作者重复了图7C所示的实验，并提供了该图的原始数据和其他量化信息。定量分析不属于原始出版物；然而，编辑认为，这种分析对于验证代表性图像的选择至关重要。校正后的图1和图7以及分析方法如下所示。原始数据和分析均包含在支持信息中。图7中的重复实验验证了本文的结论。
输注DCreg可减轻T细胞介导的气道炎症。通过腹腔注射吸附到明矾佐剂中的鸡OVA免疫小鼠（第0天）。七天后，再次免疫所有小鼠。第二次免疫后6天静脉输注PBS，imDC和DCreg。第一次免疫后第14-20天，每天将小鼠暴露于雾化的PBS或OVA中。（AB）最后一次气雾剂攻击后24小时进行BAL。（A）使用血细胞计数器计数总细胞。用Wright-Giemsa对BAL细胞的Cytospin制剂进行染色，以确定差异细胞计数。数据表示为平均值+SD（每个实验n = 6只小鼠），并且来自代表进行三个实验的单个实验。数据分析使用两尾学生t检验进行；* p <0.05（B）通过ELISA测定BAL液体上清液中IL-4和IL-5的浓度。数据表示为平均值+SD（每个实验n = 6只小鼠），并且来自代表进行三个实验的单个实验。数据分析使用两尾学生t检验进行；* p ＜0.05。（C）第一次免疫后21天，从小鼠中取肺，并通过计数靠近气道的核数来量化炎症。图像（上图）代表每个组，下图是量化。核计数的定量数据以箱形图表示，中位数由每个箱形线表示。离群值由圆圈表示。数据来自单个实验，代表每组/每个实验用5-6只小鼠进行的三个实验。数据分析使用方差分析和Tukey的多重比较测试进行；** p ＜0.01。
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