Fresh leaf vegetables are a significant part of the Persian food. Following a survey for identification of nanoviruses and geminivirus infecting leaf vegetables, a novel geminivirus was identified in a diseased parsley sample showing upward marginal leaf curling, marginal leaf yellowing, dwarfing and reduced leaf size in south-eastern Iran. The genome was identified through combination of rolling circle amplification (RCA) and high throughput sequencing (HTS) approaches. The full-length genome (2779 nts) of the cloned geminivirus, parsley yellow leaf curl virus (PYLCV), shares <66 % genome-wide pairwise identity with all other known geminiviruses. The PYLCV genome has six open reading frames (ORFs) and appears to be a hybrid with the virion sense encoded proteins being most similar to those of becurtoviruses and curtoviruses, whereas the complementary sense encoded proteins are most similar to those of begomoviruses. In comparison with other geminivirus encoded capsid proteins (CPs) and replication associated proteins (Reps), the CP of PYLCV shares <56 % amino acid pairwise identity whereas the Rep shares <73 % amino acid pairwise identity. To demonstrate the pathogenicity of the geminivirus, a partial dimer infectious clone was constructed and used to agro-infect parsley as well as Nicotiana benthamiana, turnip, radish and tomato. The agro-inoculation resulted in infection with symptoms in 83.7 % (82/98) of the tested plant. Based on the similarity of the CP encoded by PYLCV to those of becurtoviruses and curtoviruses, it is likely that leafhoppers may be the primary transmission vector.

新鲜的蔬菜叶是波斯食物的重要组成部分。在进行了一项针对感染叶菜的纳米病毒和双生病毒的鉴定调查之后，在患病的欧芹样品中鉴定出一种新型双生病毒，表现出伊朗东南边缘的叶片向上卷曲，边缘变黄，变矮并且叶片尺寸减小。通过滚动环扩增（RCA）和高通量测序（HTS）方法的组合鉴定了基因组。克隆的双生病毒，欧芹黄叶卷曲病毒（PYLCV）的全长基因组（2779 nts）与所有其他已知双生病毒共享<66％的全基因组配对身份。PYLCV基因组有六个开放阅读框（ORF），并且似乎是与病毒编码的蛋白质最相似的融合蛋白，与前轮状病毒和轮状病毒相似，而互补的有义编码蛋白与begomovirus的蛋白最相似。与其他双生病毒编码的衣壳蛋白（CP）和复制相关蛋白（Reps）相比，PYLCV的CP共享<56％的氨基酸成对同一性，而Rep共享<73％的氨基酸成对同一性。为了证明双生病毒的致病性，构建了部分二聚体感染性克隆并用于农业感染香菜和烟草（Nicotiana benthamiana），萝卜，萝卜和番茄。农业接种导致受试植物感染率为83.7％（82/98）。基于由PYLCV编码的CP与前弯道病毒和弯道病毒的相似性，叶蝉可能是主要的传播载体。