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Evidence of exposure to zoonotic flaviviruses in zoo mammals in Spain and their potential role as sentinel species.
Veterinary Microbiology ( IF 3.3 ) Pub Date : 2020-06-25 , DOI: 10.1016/j.vetmic.2020.108763
J Caballero-Gómez 1 , D Cano-Terriza 2 , S Lecollinet 3 , M D Carbonell 4 , R Martínez-Valverde 5 , E Martínez-Nevado 6 , D García-Párraga 7 , S Lowenski 3 , I García-Bocanegra 2
Affiliation  

A serosurvey was carried out to assess emerging flavivirus exposure in zoo mammals in Spain and to determine the dynamics of seropositivity in species that were longitudinally sampled during the study period. Sera from 570 zoo animals belonging to 120 mammal species were collected at ten zoos (A–J) in Spain between 2002 and 2019. Twenty-one of these animals, belonging to ten different species, were sampled longitudinally at four of the zoos during the study period. Antigenically-related flavivirus antibodies were detected in 19 (3.3 %; 95 %CI: 2.0–5.2) of the 570 animals analyzed using bELISA. Seropositivity was observed in ten (8.3 %) of the 120 species tested. Five (23.8 %) of the 21 animals sampled more than once presented seropositivity in all samplings whereas seroconversion was only observed in one white rhinoceros (Ceratotherium simum). Flavivirus antibodies were found at six of the ten sampled zoos and in consecutive years between 2008 and 2018. Virus neutralization tests confirmed West Nile virus (WNV), Usutu virus (USUV) and tick-borne encephalitis virus (TBEV) infection in ten (1.8 %; 95 %CI: 0.7–2.8), five (0.9 %; 95 %CI: 0.1–1.6) and one (0.2 %; 95 %CI: 0.0−0.5) animal, respectively. Antibodies against Meaban virus (0 %; 95 %CI: 0.0−0.7 %) were not found in the tested sera. The results demonstrate WNV, USUV and TBEV exposure in zoo mammals, which may be of public health and conservation concern. Seropositivity to WNV and USUV was detected in regions where these viruses have not been reported previously. Anti-WNV antibodies found in zoo animals sampled in 2009 point to WNV circulation at least one year before the first outbreaks were reported in horses and humans in Spain. Our results indicate that zoo mammals could be useful sentinel species for monitoring emerging flavivirus activity in urban areas.



中文翻译:

在西班牙动物园的哺乳动物中暴露于人畜共患性黄病毒的证据及其作为前哨物种的潜在作用。

进行了一项血清调查,以评估西班牙动物园哺乳动物中出现的黄病毒新暴露,并确定研究期间纵向取样的物种的血清阳性反应的动态。在2002年至2019年之间,从西班牙的十个动物园(A–J)收集了来自570种动物园动物的血清,这些动物属于120种哺乳动物。在此期间,对其中的二十一种动物(属于十种不同物种)进行了纵向采样。学习时段。使用bELISA分析的570只动物中有19只(3.3%; 95%CI:2.0-5.2)检测到了抗原相关的黄病毒抗体。在120种受试物种中,有十种(8.3%)观察到了血清阳性。在21只动物中,有五只(23.8%)在所有采样中均出现了血清阳性,而仅在一只白犀牛中观察到了血清转化(白灵藻)。在十个动物园中的六个中以及在2008年至2018年之间的连续几年中发现了黄病毒抗体。病毒中和测试确认了十个(1.8中)感染了西尼罗河病毒(WNV),乌苏图病毒(USUV)和tick传脑炎病毒(TBEV) %; 95%CI:0.7-2.8),五只(0.9%; 95%CI:0.1-1.6)和一只(0.2%; 95%CI:0.0-0.5)动物。在测试的血清中未发现针对Meaban病毒的抗体(0%; 95%CI:0.0-0.7%)。结果表明,WNV,USUV和TBEV在动物园哺乳动物中的暴露,可能是公共卫生和保护关注的问题。在以前未报告过这些病毒的区域中检测到了对WNV和USUV的血清阳性。在2009年采样的动物园动物中发现的抗WNV抗体指出,在西班牙首次爆发马和人类暴发前一年,WNV流行。

更新日期:2020-06-28
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