Glutathione (GSH) is an important biothiol in biological systems and serves as a significant biomarker for the health of humans. Herein, red-emitting carbon dots (p-CDs) prepared by a simple one-step solvothermal process can exclusively, rapidly and sensitively respond to GSH owing to the high content of pyridinic N. Upon addition of GSH, p-CDs instantly give a significant color change from orange to bluish-purple. The absorbance ratio of 576 nm to 486 nm is linearly proportional to the logarithmic concentration of GSH in a wide range of 12.5−800 μM, which is the level of cellular GSH. Meanwhile, the fluorescence of p-CDs quenches obviously. The LOD of p-CDs towards GSH in fluorescence mode is 0.7 μM. Moreover, p-CDs have no response to Cys, Hcy, amino acids and ions existing in biological systems. Such a promising responsiveness offers a great opportunity to accurately, sensitively and selectively detect GSH by colorimetric and fluorescent dual modes. In addition, cellulose/p-CDs hydrogels and p-CDs-based test papers have been fabricated to successfully detect GSH according to the color-change and fluorescence quenching. The biocompatible, environment-friendly and disposable cellulose/p-CDs materials readily provide a simple and visual strategy to detect GSH levels in the biomedical fields.

谷胱甘肽（GSH）是生物系统中重要的生物硫醇，并且是人类健康的重要生物标志物。在此，由于吡啶氮N的含量高，通过简单的一步溶剂热法制备的红色发光碳点（p-CD）可以专有，快速且灵敏地响应GSH。添加GSH时，p-CD立即产生颜色从橙色显着变为蓝紫色。576 nm至486 nm的吸光度比与GSH的对数浓度线性相关，在12.5-800μM的宽范围内，这是细胞GSH的水平。同时，p-CDs的荧光猝灭。在荧光模式下，p-CD对GSH的LOD为0.7μM。此外，p-CD对生物系统中存在的Cys，Hcy，氨基酸和离子无反应。如此有希望的响应性提供了通过比色和荧光双重模式准确，灵敏和选择性地检测GSH的巨大机会。此外，已经制造了纤维素/ p-CDs水凝胶和基于p-CDs的试纸，以根据颜色变化和荧光猝灭成功检测GSH。具有生物相容性，环境友好和一次性的纤维素/ p-CDs材料可轻松提供简单直观的策略来检测生物医学领域中的谷胱甘肽水平。