Abstract The applicative potentiality of the nanotechnologies emerges more and more in all fields of science, technology, and medicine. In this context, magnetic nanoparticles have been recently proposed as promising nanomaterials to eliminate, through minimally invasive treatments, small tumours. This work deals with the design and realization of electrospun hybrid membranes of Polycaprolactone (PCL) incorporating magnetic nanoparticles modified with the acid citric-based ligand. The nature of the ligand allows very effective compatibility between nanoparticles and hosting fibers, as deducible by the nanoparticles distribution along the fibers. Dimension and functionalization of the nanoparticles, together with the optimization of the electrospinning parameters allow obtaining highly homogeneous distribution in the diameter of the nanocharged fibers, which ranges between 500 nm and 3 μm. The anticancer activity of the optimized electrospun hybrid membranes has been analyzed toward two different melanoma cell lines: the low metastatic A375 and the high metastatic A2058, using the MTT assay. The formulated membrane exhibited a dose-dependent reduction toward both melanoma cells viability, without a decrease of activity compared to the unfunctionalized Fe3O4 nanoparticles. Promising results have been obtained also considering the antitumor activity of the hybrid Membranes toward uterine HeLa cells. Magnetic properties and structural and morphological characteristics of the functional membranes highlight very promising applications for tuning/enhancing the nanodelivery of drugs and chemotherapy assisted by electroporation technique.

中文翻译：

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抗肿瘤电纺聚己内酯/功能化Fe3O4杂化膜的开发和表征

摘要 纳米技术的应用潜力越来越多地出现在科学、技术和医学的各个领域。在这种情况下，磁性纳米粒子最近被提出作为有前途的纳米材料，通过微创治疗消除小肿瘤。这项工作涉及聚己内酯 (PCL) 电纺混合膜的设计和实现，其中包含用酸性柠檬酸基配体修饰的磁性纳米粒子。配体的性质允许纳米颗粒和宿主纤维之间非常有效的相容性，正如纳米颗粒沿纤维分布所推断的那样。纳米颗粒的尺寸和功能化，结合静电纺丝参数的优化，可以在纳米带电纤维的直径范围内获得高度均匀的分布，范围在 500 nm 和 3 μm 之间。已使用 MTT 测定分析了优化的电纺混合膜对两种不同黑色素瘤细胞系的抗癌活性：低转移性 A375 和高转移性 A2058。与未官能化的 Fe3O4 纳米颗粒相比，配制的膜对两种黑色素瘤细胞的活力均呈剂量依赖性降低，而活性没有降低。考虑到杂交膜对子宫 HeLa 细胞的抗肿瘤活性，也获得了有希望的结果。