当前位置: X-MOL 学术Mater. Des. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
In vitro and in vivo evaluation of Ca/P-hyaluronic acid/gelatin based novel dental plugs for one-step socket preservation
Materials & Design ( IF 8.4 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.matdes.2020.108891
Hoe-Jin Kang , Seong-Su Park , Tarek Saleh , Kang-Min Ahn , Byong-Taek Lee

Abstract Teeth extraction is inevitable in the case of progressive or alveolar bone destructing chronic periodontitis. Unfortunately, the resulting bone defects may lead to many side effects. Here, we developed a novel dental-bone substitute to preserve the extraction socket for hemostasis and bone regeneration. Hyaluronic acid-gelatin hydrogel (HG) polymers, β-tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP) ceramics were fabricated using freeze-drying methods. The HG/TCP/BCP plug was prepared to be easily applied by pouring the mixture of HG and TCP into the shell-shaped mold, followed by placing the sponge type of BCP in the core portion of the mold compared to HG/TCP and collagen plugs. In vitro studies showed that HG/TCP and HG/TCP/BCP plugs were cytocompatible and could promote osteogenesis by upregulating the expressions of bone-related genes COL1, RUNX2, ALP, and OPN. The rabbit-femur defect model revealed that the implanted HG/TCP/BCP plug, which showed excellent hemostatic property, promote bone regeneration with a high rate of collagen distribution along with ALP and OPN expressions more than could the HG/TCP plug at 3 months after implantation, whereas the collagen plugs just filled with chondrocytes for cartilages formation. The HG/TCP/BCP plug should be considered for potential dental applications for one-step socket preservation.

中文翻译:

基于Ca/P-透明质酸/明胶的新型牙塞用于一步牙槽窝保存的体外和体内评价

摘要 在进行性或牙槽骨破坏性慢性牙周炎的情况下,拔牙是不可避免的。不幸的是,由此产生的骨缺损可能会导致许多副作用。在这里,我们开发了一种新型的牙骨替代品,以保留拔牙窝以进行止血和骨再生。透明质酸-明胶水凝胶 (HG) 聚合物、β-磷酸三钙 (TCP) 和双相磷酸钙 (BCP) 陶瓷是使用冷冻干燥方法制造的。通过将 HG 和 TCP 的混合物倒入壳形模具中,然后将海绵型 BCP 放置在模具的核心部分,与 HG/TCP 和胶原蛋白相比,制备了 HG/TCP/BCP 塞以使其易于应用插头。体外研究表明,HG/TCP 和 HG/TCP/BCP 塞具有细胞相容性,可以通过上调骨相关基因 COL1、RUNX2、ALP 和 OPN 的表达来促进成骨。兔股骨缺损模型显示,植入的 HG/TCP/BCP 塞具有良好的止血性能,在 3 个月时比 HG/TCP 塞更能促进骨再生,胶原分布以及 ALP 和 OPN 表达率高植入后,而胶原蛋白塞只是填充了软骨细胞以形成软骨。HG/TCP/BCP 插头应考虑用于潜在的牙科应用,以进行一步式牙槽窝保存。在植入后 3 个月时,与 HG/TCP 塞相比,以高胶原蛋白分布以及 ALP 和 OPN 表达促进骨再生,而胶原塞只是填充了软骨细胞以形成软骨。HG/TCP/BCP 插头应考虑用于潜在的牙科应用,以进行一步式牙槽窝保存。在植入后 3 个月时,与 HG/TCP 塞相比,以高胶原蛋白分布以及 ALP 和 OPN 表达促进骨再生,而胶原塞只是填充了软骨细胞以形成软骨。HG/TCP/BCP 插头应考虑用于潜在的牙科应用,以进行一步式牙槽窝保存。
更新日期:2020-09-01
down
wechat
bug