Fluorescence measurements of the marine flatworm Macrostomum lignano were performed during exposure to the explosive TNT and its main derivatives 2-ADNT and 4-ADNT, using calcein AM, the acetoxymethylester of calcein, and the autofluorescence of its food (diatoms). Lethality was found to depend on temperature and exposure time. After 12 days of exposure to a concentration of 33,3 mg/L 2-ADNT and 4-ADNT, the lethality at 30 °C (100%) was strongly increased compared to 21 °C (~60%). First deaths were observed after four days of exposure. Using lower concentrations (≤3,33 mg/L) of all three compounds, the activity of ABC transporters (ATP binding cassette transporter) was determined using calcein as reporter dye. Worms exposed to toxicants for 72 h showed a significant upregulation of ABC transporter activity during exposure to 3,33 mg/L 2-ADNT and 4-ADNT, and 3 mg/L TNT demonstrating the efficacy of this cellular first line defense. A distinct behavioral defense of the worms decreased the uptake of 2-ADNT and 4-ADNT (0,033 mg/L) as they reduced feeding shown by diminished autofluorescence of algae in the gut.

海洋扁虫Macrostomum lignano的荧光测量在暴露于炸药TNT及其主要衍生物2-ADNT和4-ADNT的过程中，使用钙黄绿素AM，钙黄绿素的乙酰氧基甲基酯及其食品（硅藻）进行自发荧光。发现致命性取决于温度和暴露时间。暴露于33,3 mg / L 2-ADNT和4-ADNT浓度的12天后，与21°C（〜60％）相比，在30°C（100％）时的致死率大大提高。暴露四天后观察到首次死亡。使用所有三种化合物的较低浓度（≤3.33mg / L），使用钙黄绿素作为报告染料测定ABC转运蛋白（ATP结合盒转运蛋白）的活性。暴露于有毒物质72小时的蠕虫在暴露于3.33 mg / L的2-ADNT和4-ADNT期间显示出ABC转运蛋白活性的显着上调，和3 mg / L TNT证明了这种细胞一线防御的功效。蠕虫的独特行为防御功能降低了2-ADNT和4-ADNT（0.033 mg / L）的摄取，因为它们减少了肠道藻类的自发荧光，从而减少了进食。